a high speed detection platform based on surface-enhanced raman scattering for monitoring antibiotic-induced chemical changes in bacteria cell wall基于表面增强拉曼散射的高速检测平台监控antibiotic-induced细菌细胞壁的化学变化.pdfVIP

A High Speed Detection Platform Based on Surface- Enhanced Raman Scattering for Monitoring Antibiotic- Induced Chemical Changes in Bacteria Cell Wall 1 2 3 2 3 2 Ting-Ting Liu , You-Hsuan Lin , Chia-Sui Hung , Tian-Jiun Liu , Yu Chen , Yung-Ching Huang , Tsung- 4 5,6 4 5,7 5,6 Heng Tsai , Huai-Hsien Wang , Da-Wei Wang , Juen-Kai Wang *, Yuh-Lin Wang *, Chi-Hung Lin2,3,8,9* 1 Division of Research and Development, National Yang-Ming University, Taipei, Taiwan, 2 Institute of Biophotonics, School of Biomedical Science and Engineering, National Yang-Ming University, Taipei, Taiwan, 3 Institute of Microbiology and Immunology, School of Life Science, National Yang-Ming University, Taipei, Taiwan, 4 Institute of Information Science, Academia Sinica, Taipei, Taiwan, 5 Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, Taiwan, 6 Department of Physics, National Taiwan University, Taipei, Taiwan, 7 Center for Condensed Matter Sciences, National Taiwan University, Taipei, Taiwan, 8 Taipei City Hospital, Taipei, Taiwan, 9 Taipei Veteran General Hospital, Taipei, Taiwan Abstract Rapid and accurate diagnosis for pathogens and their antibiotic susceptibility is critical for controlling bacterial infections. Conventional methods for determining bacterium’s sensitivity to antibiotic depend mostly on measuring the change of microbial proliferation in response to the drug. Such ‘‘biological assay’’ inevitably takes time, ranging from days for fast- growing bacteria to weeks for slow-growers. Here, a novel tool has been developed to detect the ‘‘c