a closer look at the eve stripe 2 enhancers of drosophila and themira仔细看看前夕条纹2增强果蝇和themira.pdfVIP

Perspective A Closer Look at the eve Stripe 2 Enhancers of Drosophila and Themira Justin Crocker, Albert Erives* Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire, United States of America Introduction conclusions addressing an important on- ments. We also computed the same dot going debate on the degree of functional plot using the reverse complement of one Gene regulatory sequences have been organization of enhancers [29]. The of the sequences (Figure 1B and 1E). In investigated and/or proposed to be im- results of this debate therefore impact the addition to showing similar sequences that portant targets of natural selection during much larger discussion on the genetic loci happen to occur in the opposite orienta- animal evolution [1–14]. However, much of evolution [15,16]. tion, graphing the reverse complement controversy has been generated by the Both Drosophila and Themira are acalyp- serves as an internal negative control for contention that they are not likely to be as trate flies and last shared a common ancestor conservation of serial blocks of sequence. important as functional protein-coding at least ,110 Mya, and so it is suggested Here, we report that when we graph the evolution given the low number of such that this distance is long enough for the eve stripe 2 enhancers in parallel orienta- examples established to date [15,16]. sequences to be completely scrambled in tions, we see large blocks of alignment However, an important