土壤中砷氧化菌的生理生化及转化砷特性研究.PDF

, JournalofAnhuiAgr.i Sci. 2011, 39(6): 3397- 3399, 3418 刘月娟 马君叶 * 宋卫锋, 罗丽丽 , 林梓河, 严 明, 邓琪, 莫于婷 (, 510006) [目的]通过外加砷源驯化肇庆市鼎湖山 然保护区土壤中细菌,研究砷氧化菌的生理生化及转化砷特性[方法]采用富集稀 释平板硝酸盐漫过生理生化指标的测定等[结果]从中分离鉴定出具有氧化砷功能的产碱杆菌和土壤杆菌 2种菌株[结论]这 2 种菌株最适氧化砷温度为 30e , 最适氧化砷 pH为 9培养基中乳酸钠浓度对菌株氧化砷有一定的影响 土壤;砷氧化菌;特性 Q 93- 33 A 0517- 6611(2011)06- 03397- 03 Arsenite Transformation CharacteristicsandMolecularIdentification ofArsenic-oxidizingBacteria Isolated from Soil SONG Wei-feng et al (College of Environ ental Science and Engineering, Guangdong University of Technology, Guangzhou, Guangdong 510006) Abstract [Objective]Through do esticated bacteria fro the applied arsenic source soil inDinghushanNatureReserve ofZhaoqing City, phys-i ological, bioche icaland transfor ation characteristicsofarsenic oxide bacteriaw ere studied. [M ethod]The ethodsof concentration, platesser- ial dilution, silver nitrate overflowed, physiological characteristicwere adopted. [Result] They were identified as alcaligenes castellani and agrobacteriu conn respectively, whichwere able to oxidize arsenite (As( III) ) into arsenate (As(V) ). [Conclusion]The opti al te perature and pH were 30e and 9 respectively for two bacterialstrains. In addition, sodiu lactate ediu concentration had a certain i pact to arsenic- oxidizing. eywords soil; Arsenic-oxidizing bacteria; Characteristic (As) 33,, [ 1] , , 1918 [2] (MMAA)( DMAA) (arsenite-oxidizing bacteria), 9 As, [ 10] 30 , [ 3] , 0. 1~ 9. 0