响应面优化酶法提取平菇多糖工艺探究.docVIP

响应面优化酶法提取平菇多糖工艺探究（1.浙江中医药大学,浙江 杭州 310053；2.浙江中医药大学附属第一医院,浙江 杭州 310007） 摘 要：目的:建立平菇多糖的含量测定方法，确定纤维素酶法提取平菇多糖的最佳工艺条件。方法:采用苯酚-硫酸法测定平菇多糖的含量。以平菇多糖得率为指标，考察酶解时间、酶解温度、pH值等3个因素对平菇多糖纤维素酶提取工艺的影响，采用响应面分析法优化工艺条件。结果:平菇多糖的最佳提取工艺条件为酶解时间135min、酶解温度49℃、pH5.30，在此条件下得率可达6.82%。结论 该法成本低、产品得率高，比传统水提法提高3.98%。 关键词：纤维素酶法；平菇；多糖；响应面分析法 中图分类号：R284.2文献标识码：A文章编号：1673-7717（2011）03-0650-04 Optimization of Enzymatic Extraction Technology of Polysaccharides from Pleurotus ostreatus by Response Surface Methodology QIAN Lei1,ZHANG Chun-chun1,JIANG Jian-ping1,2,XIONG Yao-kang1 （1.Zhejiang Chinese Medical University,Hangzhou 310053,Zhejiang,China； 2.The Frist Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310007,Zhejiang,China） Abstract:Objective:To establish determination methods and optimal extraction conditions of polysaccharides from Pleurotus ostreatus treated with cellulase.Methods:Total carbohydrate content was determined by the phenol-sulfuric acid method and the yield of polysaccharides was the indice to determine extraction process by a combination of response surface methodology （RSM）.the enzymolysis time,enzymolysis temperature and pH value were selected as factors and a three-factors-three-levels experiment was developed by Box-Benhnken central composite design method.Results:The optimal conditions of variables were determined as follows:enzymolysis time 139 min, enzymolysis temperature 49℃ and pH value 5.3.Under the condition above, the yield of polysaccharides was 6.82%.Conclusion:It was a ideal means with relative low cost and higher extraction efficiency of polysaccharides,which could be improved by 3.98 % through the treatment with cellulase than without. Key words:cellulase;Pleurotus ostreatus;polysaccharides;response surface methodology 收稿日期：2010-10-08 作者简介：钱磊（1985-），男，浙江嵊州人，硕士研究生，研究方向：中药资源的开发与利用。 通讯作者：熊耀康（1959-），男，浙江衢州人，教授，博士研究生导师，研究方向：中药资源的开发与利用。 平菇为担子菌亚门、层菌纲、伞菌目、口磨科平菇（ Pleurotus ostreatus）的子实体，又名白平菇、侧耳，是广泛栽培的食用菌之一