VEGF明胶缓释微球对大鼠背部随意皮瓣存活影响.docVIP

VEGF明胶缓释微球对大鼠背部随意皮瓣存活影响[摘要]目的：研究局部注射血管内皮生长因子(VEGF)复合明胶微球对SD大鼠背部随意皮瓣存活的影响。方法: 采用改良的乳化冷凝法交联制备复合VEGF 的明胶缓释微球,将其注射于大鼠背部随意皮瓣，24只SD大鼠随机分为复合VEGF微球组（A组）、VEGF治疗组（B组）和对照组（C组），术后7天分别进行皮瓣存活率、新生血管计数的检测。结果：术后7天皮瓣的存活率分别为(68.54±2.79)%,(58.65±3.26)%,（45.43±2.71）%,治疗组存活率显著高于对照组（P＜0.05），且存活质量A组最好；皮瓣内新生血管计数分别为(31.16±4.38),(25.41±4.06),(18.68±5.44)具有显著差异性。结论:VEGF缓释微球可以促进缺血皮瓣的血管新生,提升皮瓣存活率。 [关键词]血管内皮生长因子；微球；明胶；缓释；随意皮瓣；存活 [中图分类号]R622[文献标识码]A[文章编号]1008-6455（2008）05-04 The efect of VEGF-impregnated microsphere system on random dorsal skin flap survival in rat WANG Lu,GUO Shu-zhong,MA Xian-jie,SONG Bao-qiang,WU Shang-min,YI Cheng-gang (Institute of Plastic Surgery of People’s Liberation Army, Xijing Hospitol,the Fourth Military Medical University,Xian 710032) Abstract: ObjectiveTo study the effect of VEGF sustained release microsphere system on survival of the ischemic skin flap in rats．MethodsThe VEGF - impregnated microsphere was prepared by emulsified cold -condensation method and twenty-four SD rats were randomized into three groups with 8 rats each. An ischemic skin flap s of 8 cm ×2 cm were designed on the back. Rats received subdermal injection of the VEGF - impregnated microsphere ( group A) , VEGF (group B) and saline(group C). The survival rate of the flap and the number of the new bloods vessels were determined on the 7th day after operation. ResultsThe flap survival rate were(68.54±2.79)%, (58.65±3.26) %, (45.43±2.71)%respectively,with significant between any 2 group( all P＜0.05) and the survival quality of the group A was the best. And the number of the new blood vessels were(31.16±4.38),(25.41±4.06),(18.68±5.44)respectively.there was significant difference statistically.CondusionThe VEGF - impregnated microsphere can increases the neovascularization in ischemic skin flaps and augments their survival rate. Key words: vascular endothelial grouth factors(VEGF);microsphere,gelatin;sustained-release;random pattern flap;survial 应用皮瓣进行创面修复、功能重建及外形改善是整形外科最常用的治疗方法