胰岛素样生长因子Ⅰ对组织工程肌腱细胞增殖影响的量效关系.PDF

12 2 Journal of Clinical Rehabilitative Tissue Engineering Research January 8, 2008 Vol.12, No.2 1 2 3 3 3 Dose-effect relationship of insulin-like growth factor and the proliferation of tenocytes in tissue engineering 1 2 3 3 3 Qiu Nan-hai , Xia Ying-peng , Li Ming-xin , Li Rui-hua , Kan Shi-lian Abstract AIM: Insulin-like growth factor (IGF- ) is a potent mitogen and powerful stimulator for the division and growth of tenocytes. In 1Department of this study, we cultured the tenocytes of the second generation with IGF- at different doses to verify the effects of IGF- on cell Orthopedics, Tianjin Haihe Hospital, proliferation and the dose effect. Tianjin 300350, METHODS: The experiment was performed in the laboratory of Tianjin Hospital from November 2004 to April 2005. 200 China; 2Tianjin France Lohmann fertilized eggs were provided by the laboratory of Tianjin Hospital. On day 19, the tendons of 10 embryo chickens Peoples Hospital, were obtained. Tenocytes were isolated and cultured. The cell morphous and growth rules were observed. The second generation Tianjin 200121, China; 3Tianjin cells were seeded onto 6-well culture plate and added with fetal calf serum (FCS) of different concentrations. The effects of serum Hospital, Tianjin concentration on cell attachment and growth were observed. The second generation tenocyt