《酶工程》试题二答案（Enzyme engineering examination questions two answers）.docVIP

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《酶工程》试题二答案（Enzyme engineering examination questions two answers）.doc

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《酶工程》试题二答案（Enzyme engineering examination questions two answers）

《酶工程》试题二答案（Enzyme engineering examination questions two answers） Enzyme engineering examination questions two answers First, fill in the blanks (1 points per minute, 28 points) 1, Japan called enzyme things, Chinese called enzyme, English is Enzyme, is the first use of Kuhne (1878). In fact, it exists in the cells of the organism and outside the cells. 2 and 1926, Sumner (Sumner) first made urease crystallization, and pointed out that the essence of enzymes is protein. For his outstanding contribution, he won the Nobel prize for chemistry in 1947. 3. The high yield sugar ratio enzyme is widely used in our country. The strain number is As3.4309, the high yield liquefying enzyme is fine, and the strain number is BF7.658. In the microbial classification, the former belongs to the mold, and the latter belongs to bacteria. 4, 1960, check Cooper (Jacob) and Moroder (Monod) proposed that DNA operon, molecular, and related enzyme biosynthesis genes have four kinds, namely genetic manipulation and regulating gene, promoter and structural gene. 5, 1961, the International Commission on enzyme activity unit of enzyme required: under certain conditions (25oC, PH and substrate concentration is the most suitable) every 1 minutes, 1 mol catalytic amount of enzyme substrate into product as an international unit, namely 1IU. 6. The main purpose of enzyme molecule modification is to improve the enzyme performance, which is to improve enzyme activity, decrease antigenicity and increase stability. 7. The production methods of enzyme are extraction, fermentation and chemical synthesis. 8. By means of bifunctional reagents to make cross-linking between enzyme molecules, the method of making immobilized enzyme with reticular structure is called cross-linking method. 9 、 in the method of enzyme separation and purification, there are three kinds of gel filtration method, ultrafiltration method and ultra centrifugal method according to the size difference between the target enzyme and the imp