a6 d-dimer金标法检测操作规程（A6 D-dimer gold standard test procedure）.docVIP

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a6 d-dimer金标法检测操作规程（A6 D-dimer gold standard test procedure）.doc

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a6 d-dimer金标法检测操作规程（A6 D-dimer gold standard test procedure）

a6 d-dimer金标法检测操作规程（A6 D-dimer gold standard test procedure） A6 D-DIMER gold standard method for the detection of operating procedures A6.1 home page D-DIMER gold standard method for the detection of operating procedures (NycoCard Reader II gold standard monitor) A6.2 detection principle Based on the principle of immune filtration test, the plasma will be added detection plate hole, sample is inhaled, D-Dimer molecules by D-Dimer monoclonal antibody coated membrane capture, conjugate solution and then add the D-Dimer monoclonal antibody and colloidal gold film, D-Dimer and gold labeled antibody binding reaction, the formation of double antibody sandwich chimera the excess of gold labeled antibody with liquid detergent wash, use NycoCard ReaderII detection film Red intensity proportional to the content of D-Dimer in plasma and red intensity. A6.3 detection project D-Dimer determination of content. A6.4 sample collection and treatment A6.4.1 NycoCard analyzer using venous blood plasma analysis. A6.4.2: unless the venous blood in the blood for bedridden patients, generally take seat, position effect of water distribution in the capillaries. Take blood from elbow vein, infants blood taken from the jugular vein and femoral vein. Time is not more than 1min using tourniquet, puncture immediately after the successful release of the tourniquet, when need to use tourniquet should choose another arm. (1): venous blood collection blood apparatus apparatus using 3.2% sodium citrate anticoagulant vacutainer and matched with the No. 7 sterile needle. The blood and anticoagulant ratio 9:1. (2) sampling steps: operating in strict accordance with the standard procedures for venous blood sampling. Blood after going to flip vacuum blood anticoagulation. Flip to prevent most blood blood clots or platelet emboli 8 to 10 times. A6.5 Kit The laboratory of Norway NycoCard Reader II multifunctional quantitative gold standard monitor reagents, authorized by the Norway Tianjin City Jia Shihlin