721分光光度计的标准操作程序（Standard operating procedure for 721 Spectrophotometer）.docVIP

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721分光光度计的标准操作程序（Standard operating procedure for 721 Spectrophotometer）.doc

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721分光光度计的标准操作程序（Standard operating procedure for 721 Spectrophotometer）

721分光光度计的标准操作程序（Standard operating procedure for 721 Spectrophotometer） 721 spectrophotometer standard operating procedures Post By:2007-1-21 10:34:00 [Objective] The correct use and maintenance of 721 spectrophotometer. Change the SOP file [] In strict accordance with the instrument instructions set. [procedures] 1. open the instrument power switch, open the cuvette box cover, adjust the potentiometer knob 0, the meter pointer is light transmittance (T) of 0, up about 20 minutes. 2. to adjust the wavelength (lambda) regulating knob, monochromatic light with wavelength selection. 3. sensitivity adjustment switch, select the appropriate sensitivity. By adjusting knob to 0 school 0 bit transmission rate meter. 4. the cuvette box lid, the reference solution (blank) into the optical path, the clockwise rotation of the 100% potentiometer regulating knob meter pointer is the transmittance of 100%. 5. in this way for several times to adjust the transmittance of 0 and 100, until the same, can be measured. 6. the calibration solution and the test solution into the optical path, read calibration absorbance (A) value. 7. of the tested solution into the light path, read the test solution absorbance value. 8. according to the calibration solution and the test solution absorbance and concentration calculation calibration solution concentration measured. [note] 1. before using the instrument, the user should first understand the structure and work principle of the instrument, and the function of each operation knob. Before turning on the power supply, should be checked for safety instrument. 2. instruments before use to check, amplifier and monochromator two silica gel drying cylinder (at the bottom of the instrument), such as moisture change, should replace the dry blue silicone or silica gel dried and then poured out the original. 3. in the instrument has not yet been switched on, the starting position of each knob should be right. The meter pointer must be located in the 0 on t