和氨基反应荧光探针.docVIP

与氨基反应的荧光探针 Highlights of Rhodamine and TRITC Labeling Kits: Fast – label and purify antibody in about one hour Amine-specific labeling – the NHS-ester or isothiocyanate efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) Optimized procedure – following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence Single-use fluors – no need to weigh tiny amounts powder; kits contain single-use vials of reagent Efficient purification – kits include purification resin and easy-to-use spin columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery Applications for Rhodamine Labeling Reagents: Label antibodies for use as immunofluorescent probes Label oligonucleotides for hybridization probes Detect proteins in gels and on Western blots Properties of Rhodamine Dyes: Thermo Scientific Pierce Rhodamine Dyes are mixtures of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra, and for protein applications there is no need to isolate a specific isomer. TRITC is the base tetramethylrhodamine molecule functionalized with an isothiocyanate reactive group (–N=C=S) at one of two hydrogen atoms on the bottom ring of the structure. This derivative is reactive towards primary amine groups on proteins, peptides and other biomolecules. NHS-Rhodamine is activated with the N-hydroxy-succinimidyl-ester (NHS ester) functional group. Compared to TRITC, the NHS-ester deriviative has greater specificity toward primary amines in the presence of other nucleophiles and results in a more stable linkage following labeling. Texas Red* Sulfonyl Chloride is a long-wavelength derivative of rhodamine that is modified with sulfonyl chloride for reaction to primary amines. We recommend HYPERLINK /Products/Browse.cfm?fldID=9AA97866-4