用于检测强直性脊柱炎相关基因hla-b27的实时荧光pcr芯片及其检测系统.docVIP

用于检测强直性脊柱炎相关基因HLA-B27的实时荧光PCR芯片及其检测系统 ABSTRACT概要 The real-time fluorescent PCR chip and its detecting system used in examining the genes related to ankylosing spondylitis HLA-B27 ABSTRACT ? 本文介绍了我们研制的一款实时荧光PCR芯片及其检测平台。我们将生物样品的预处理与实时荧光PCR反应集成到一块芯片上。我们在芯片的腔体(2uL)内制作了一个坝，其下形成了1条1.5μm的罅缝，用于从血液中分离白细胞，然后在同一腔体中进行PCR反应。相对于常规管式PCR反应，本芯片能大大节省样品和试剂的使用量。利用微加工技术，我们还将微加热器和温度传感器集成到本芯片上。芯片的封装采用硅和玻璃的阳极键合而成。针对此芯片我们设计了相应的检测系统，它由注射泵模块、温度循环控制模块、荧光采集模块组成。将此芯片和检测系统与我公司开发的EvaGreen HLA-B27 Detection Real Time qPCR Kit（Capitalbio）相结合，进行了强直性脊柱炎相关基因HLA-B27的检测。 ? This article introduces a section of real-time fluorescent PCR chip and its testing platform developed by us. We integrated the biological samples pretreatment and the real-time fluorescent PCR reaction to one chip. We have built a dam in the chip’s cavity (2uL), and has formed a 1.5μm crack underneath it for separating the white blood cell from the blood, then carried out the PCR reaction in the identical cavity. This chip could save the amount of usage of samples and reagents compared with conventional tubular PCR reaction. Using micromachining technology, we also integrated micro-heater and temperature sensor on the chip. The material for the encapsulation of the chip is anodic bonding of silicon and glass. We have designed the corresponding examination system in view of this chip, it is composed of injection pump module, temperature cycle control module and fluorescence gathering module. We have combined the chip and testing system with EvaGreen HLA-B27 Detection Real Time qPCR Kit（Capitalbio）which developed by our company, and has carried out examination on the genes related to ankylosing spondylitis HLA-B27. ? INTRODUCTION简介 生物芯片技术是20世纪末发展起来的一项新技术。生物芯片是在微小面积上，利用微加工技术，并结合有关的化学合成技术制造而成的一种具有一定分子生物学检验功能的微型器件。分析和解释生物芯片上得到的信息，将在DNA结构与功能之间架起一道桥梁，进而推进生命科学的迅速发展。 ? Biochip is a new technology developed at the end of 20 century. Biochip, the concept of it, is using the micro micromachining technology combines with the related chemical synth