菜蛾盘绒茧蜂滞育与非滞育预蛹过氧化氢抗氧化酶及激素水平的比较.PDFVIP

28(2)165-169 中国生物防治学报 Chinese Journal of Biological Control 2012 年 5 月 菜蛾盘绒茧蜂滞育与非滞育预蛹过氧化氢、抗氧化酶 及激素水平的比较 郝仲萍，袁忠勤，张 娟，赵金瑞，施祖华* （浙江大学昆虫科学研究所，杭州 310029 ） 摘要：为了研究过氧化氢与激素在菜蛾盘绒茧蜂 Cotesia vestalis 滞育过程中的作用，本文测定了滞育与非滞 育预蛹过氧化氢的含量，3 种保护酶（SOD、POD 和 CAT ）的活性以及20E 和 JH Ⅲ的滴度，结果显示，滞 育预蛹过氧化氢含量、SOD 和 POD 活性以及 20E 滴度显著低于非滞育预蛹，而 CAT 活性与 JH Ⅲ滴度则相 反。推测高浓度的 JH Ⅲ抑制 20E 的合成和释放，20E 滴度的下降又通过调节 SOD 活性的下降和 CAT 活性的 上升来降低过氧化氢的浓度，使菜蛾盘绒茧蜂滞育。 关 键 词：菜蛾盘绒茧蜂；过氧化氢；激素；抗氧化酶；滞育 中国分类号：S476.3; Q965 文献标识码：A 文章编号：1005-9261 (2012)02-0165-05 Comparison of Hydrogen Peroxide, Antioxidant Enzymes and Hormones between Diapause and Non-diapause Prepupae of Cotesia vestalis * HAO Zhongping, YUAN Zhongqin, ZHANG Juan, ZHAO Jinrui, SHI Zuhua (Institute of Insect Sciences, Zhejiang University, Zhejiang Province, Hangzhou 310029, China) Abstract: In order to investigate the effects of hydrogen peroxide and hormones on diapause in Cotesia vestalis, hydrogen peroxide content, activities of three antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT)), and contents of 20E and JH Ⅲ between diapause and non-diapause prepupae were compared. Results showed that the hydrogen peroxide content, SOD and POD activities and 20E content in diapause prepupae were significantly lower, while the CAT activity and JH Ⅲ content were significantly higher, when compared to those in non-diapause prepupae. We speculated that high concentrations of JH Ⅲ inhibited the synthesis and release of 20E,