荆芥内酯大鼠在体胃肠吸收动力学研究-天津中草药杂志社.PDFVIP

·1384 · 中草药 Chinese Traditional and Herbal Drugs 第 42 卷 第 7 期 2011 年 7 月 荆芥内酯大鼠在体胃肠吸收动力学研究 1 1 1 1 1, 2* 耿 婷 ，朱 慧 ，张 丽 ，单鸣秋 ，丁安伟 1. 南京中医药大学，江苏 南京 210046 2. 江苏省方剂研究重点实验室，江苏 南京 210046 摘 要：目的 研究荆芥内酯在大鼠胃肠道的吸收。方法 应用大鼠在体胃肠吸收模型，采用酚红标记法校正肠循环液的体 积，HPLC 法测定不同时间点胃肠循环液中荆芥内酯的质量浓度。结果 不同质量浓度（1.84、3.68、7.36 μg/mL ）的荆芥内 酯在大鼠胃部每小时吸收率分别为（19.47±0.69 ）%、（21.66 ±1.92）%、（26.51 ±1.25）% ；在大鼠肠道的吸收速率常数（Ka ） 分别为（0.203 ±0.007 ）、（0.159 ±0.011 ）、（0.134 ±0.012 ）h−1，各组之间差异均显著（P ＜0.05 ）。结论 荆芥内酯在大鼠胃 部的吸收可能为被动转运，在肠道的吸收可能为主动转运。 关键词：荆芥内酯；在体胃肠吸收；吸收动力学；被动转运；主动转运 中图分类号：R285.61 文献标志码：A 文章编号：0253 - 2670(2011)07 - 1384 - 05 In vivo gastrointestinal absorption kinetics of schizonepetin in rats 1 1 1 1 1, 2 GENG Ting , ZHU Hui , ZHANG Li , SHAN Ming-qiu , DING An-wei 1. Nanjing University of Chinese Medicine, Nanjing 210046, China 2. Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing 210046, China Abstract: Objective To study the gastrointestinal absorption kinetics of schizonepetin in rats. Methods The drug concentration by in situ perfusion in rats was determined by HPLC and the volume of perfusion fluid was adjusted by Phenol red labeling. Results The hourly absorption percentages of three different drug concentrations (1.84, 3.68, and 7.36 μg/mL) in stomachs were (19.47 ± 0.69) %, (21.66 ± 1.92) %, and (26.51 ± 1.25) %, respectively. The absorption rate constants (K ) of three different concentrations in intestine a were (0.203 ± 0.007), (0.159 ± 0.011), and (0.134 ± 0.012) h−1, respectively, which had significant differences among them (P0.05). Conclusion Schizonepetin might be absorbed in stomachs via passive transport mechanism and in intestine via active transport mechanism. Key words: schizonepetin;