灵芝三萜hplc测定英文讲解.ppt

Figure 3. Content of 4–17 in various Linhzhi samples belong to groups A, B and C. summary The total amount of all the compounds in the wild samples was appreciably higher than in the cultivated ones. For example :lucidenic acid N (4) lucidenic acid E2 (5) The wild samples contained significantly more ganoderiol F(15) than the cultivated samples A different trend was observed for methyl lucidenate E2 (9), which was under the LOQ in the wild samples but was found in GL1, GL2, and GL3 in the range between 286.94 and 446.95 μg/g. The proportion of constituents in the other species is different from G. lucidum.such as lucidenic acid E2 (5), the amount of methyl lucidenate E2 (9), which is not observed in G. lucidum, is the major compound in the TGap, TGau, and TGc samples. It is noteworthy that the amount of the constituents in TGs was substantially smaller than others, and 50 percent was ergosterol. 1 Conclusions 4 ? This is the first time a HPLC-DAD method for quantitative analysis of constituents in Ganoderma lucidum and its related species batches originating from Vietnam was established. They have reported for the first time the presence of lanostane triterpenes, ergosterol, uracil, adenosine, and gentisic acids in the Vietnamese G. lucidum and its related species. Especially, two new lanostanes, butyl lucidenate E2 and butyl ganoderate A, were reported for the first time in G. lucidum originating from Vietnam and its four related species using a HPLC-DAD method. In addition, the highest content of methyl lucidenate E2 was found in G. australe, G. applatatum, and G. colossum, respectively. In the present study the profile of the 17 compounds differed significantly in the all analyzed samples. It can be also concluded that the geographical distributions, growth conditions, and substrates might be the key to differences in producing chemical compositions. This present work suggested an accurate and sufficient method for quantitative evaluation, whic