离子对萃取和高效液相色谱电化学检测法测定血浆中儿茶酚胺陆春苓.pdfVIP

　2004 6 28 3 Bull Acad M il Med Sci, Jun 2004;　 Vol 28　No 3　 275 - 技术方法 ＊ , , , (军事医学科学院毒物药物研究所, 北京　100850) [] 　:建立简便、灵敏的测定血浆儿茶酚胺的方法。:利用离子对萃取法提取血浆中的儿茶酚胺, 用 高效液 色谱-电化学检测法(HPLC-ECD)进行检测, 测定其回收率、线性范围、精密度及最低检测限, 并测定和比较 了大鼠和小鼠血浆中儿茶酚胺浓度。:肾上腺素(E)、去甲肾上腺素(NE)、多巴胺(DA)的加样回收率分别为 (103.2 ±4.9)%, (106.1±6.3)%和(101.1±9.0)%。日内和日间精密度分别小于14.4%和11.6%。该方法测定E 和NE 在0.5 ～16 ng 范围内, DA 在0.25 ～8 ng 范围内含量与峰高比之间呈良好的线性关系, 关系数为0.9980 ～ 0.9999, 最低检测限分别为:E 0.25 ng, NE 0.30 ng, DA 0.20 ng。大鼠血浆中E 浓度明显高于小鼠, NE 和 DA 浓度无 明显差异。:本法用于检测血浆中儿茶酚胺水平具有简便、灵敏、快速、准确的特点, 适合于小动物血浆中儿茶 酚胺浓度的测定。 [] 　离子对;高效液 色谱;电化学;儿茶酚胺类;血浆 [] 　O657.7　　　　[] 　A [] 　1000_5501(2004)03_0275_03 Determination of catecholamine levels in plasma by ion-pairs extraction and high perfor- mance liquid chromatography-electrochemical detection ＊ LU Chun -L ing , ZHOU Wen -X ia , CHEN GJ un-P ing , ZHANG Yong -Xiang (Institute of Pha macology and Toxicology, Academy of Milita y Medical Sciences, Beijing 100850, China) [Abstract] 　Objective:To develop a simple and sensitive method to dete mine catecholamine level in plasma.Methods:The catecholamine in plasma samples was ext acted by ion-pai s and dete mined by high-pe fo mance liquid ch omatog aphy with elec- t ochemical detection (HPLC-ECD).Results:The ecove y of epineph ine (E), no epineph ine (NE)and dopamine (DA)was (103.2±4.9)%, (106.1±6.3)%and (101.1±9.0)%, espectively.The int a-and inte -assay coefficients of va iation we e lowe than 14.4%and 11.6%.A good linea co elation was found between the content and atio of peak heights which we e at the ange of 0.5-16 ng fo E and NE, and 0.25-8 ng fo DA, the co elation coefficients we e at the ange of 0.9980- 0.9999.The minimum detection limit of E, NE and DA we e 0.25 ng, 0.30 ng and 0.20 ng, espectively.