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Analysis of phenols by highperformance liquid ...（分析酚类化合物的高效能的液体）.pdf

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Analysis of phenols by highperformance liquid ...（分析酚类化合物的高效能的液体）

Analytica Chimica Acta 543 (2005) 52–57 Analysis of phenols by high-performance liquid chromatography with pre-column derivatization by 2-(9-carbazole)-ethyl-chloroformate Lin Zhang, Lihua Zhang, Weibing Zhang, Yukui Zhang ∗ Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, PR China Received 2 March 2005; received in revised form 5 April 2005; accepted 9 April 2005 Available online 12 May 2005 Abstract 2-(9-Carbazole)-ethyl-chloroformate (CEOC), a novel pre-column ﬂuorescence labeling reagent, has been synthesized and applied for the derivatization of phenols. Taken phenol, p -chlorophenol, 2,5-dimethylphenol, 2,4-dichlorophenol and 1,4-dihydroxybenzene as testing standards, the effects of derivatization conditions, such as pH of borate buffer, reaction time and ﬂuorescent tagging reagent concentration, have been systematically studied. Under the optimized conditions, CEOC reacts readily with the phenols to form stable derivatives with excitation and emission wavelengths, respectively, at 293 and 360 nm. The single step derivatization reaction could be ﬁnished within 20 min even at room temperature. Such a method has been successfully applied to the analysis of phenols in printing ink by high-performance liquid chromatography. © 2005 Elsevier B.V. All rights reserved. Keywords: 2-(9-Carbazole)-ethyl-chloroformate; Fluorescence derivatization; High-performance liquid chromatography; Phenols 1. Introduction (HPLC) is also difﬁcult because they lack appropriate spectroscopic properties [14–17]. In addition, because of