玉竹挥发油超临界CO2 萃取条件及抑菌活性研究.PDFVIP

※工艺技术 食品科学 2011, Vol. 32, No. 08 155 玉竹挥发油超临界CO2萃取条件及抑菌活性研究 1 2 2 3 3 赵秀红 ，曾 洁 ，高海燕 ，孙希云 ，郑煜焱 ( 1.沈阳师范大学工程技术学院，辽宁 沈阳 110034 ；2 .河南科技学院食品学院，河南 新乡 453003 ； 3.沈阳农业大学食品学院，辽宁 沈阳 110866) 摘 要：运用超临界流体CO2 萃取技术提取玉竹挥发油。采用正交试验研究萃取温度、萃取压力、分离温度、分 离压力因素对玉竹挥发油得率的影响，以得率为主要标准，确定最佳工艺条件。结果表明：萃取温度35 ℃、萃 取压力30MP a 、分离温度20 ℃、分离压力9MP a 为最佳提取工艺。通过抑菌试验发现，玉竹挥发油对细菌、霉 菌、酵母菌、放线菌均有一定的抑菌活性，并具有良好的热稳定性。 关键词：玉竹；挥发油；超临界C O 2 萃取；抑菌；热稳定性 Supercritical CO2 Extraction and Antimicrobial Activity Evaluation of Polygonatum odoratum Essential Oil 1 2 2 3 3 ZHAO X iu -h on g ，ZENG Jie ，GA O Hai-yan ，SUN Xi-yun ，ZHEN G Yu -y an (1. College of Engineering and Technology, Shenyang Normal University, Shenyang 110034, China ； 2. School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, China ； 3. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China) ：In this study, supercritical fluid CO2 extraction was applied to extract Polygonatum odoratum essential oil. The effects Abstract of extraction temperature and pressure as well as separation temperature and pressure on oil yield were investigated in single factor experiments and further, the extraction parameters were optimized using a 4-factor, 3-level, 9-run orthogonal array design based on oil yield. The results showed that extraction temperature of 35 ℃, extraction pressure of 30 MPa, separation temperature of 20 ℃and separation pressure 9 MPa were found optimal for the extraction of polygonatum essential oil. The oil had