基于抗氧化作用的淫羊藿抗衰老药效研究.PDFVIP

Anti-aging Effects of Epimedium Acuminatum Based on the Anti-oxidant Effect MA Shan1, MA Peipei1,2, CHEN Wenyuan1,2, CHEN Xiao1,2, DU Lina1,2*, TIAN Jingzhen1*(1.Department of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China; 2.Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing 100850, China) ABSTRACT: OBJECTIVE To verify the anti-aging function of Epimedium acuminatum (E. acuminatum) resulting from their anti-oxidation effects not from immunological effect. METHODS Aged mice were made via subcutaneous injecting D-galactose. The ethanol extract of E. acuminatum was administered orally to the aged mice. A series of biomarkers related to aging, including malonydialdehyde (MDA), superoxide dismutase (SOD), protein carbonyl (PCO), and nitric oxide (NO) were detected. Immunology indices of thymus indices and changes of T lymphocytes were determined with or without the oral administration of extracts. RESULTS E. acuminatum decreased the levels of MDA, PCO, and increased the levels of SOD and NO. This demonstrated that the anti-oxidation effect of E. acuminatum was obvious. However, it had little effects on the thymus indices, and changes of T lymphocytes. CONCLUSION E. acuminatum shows anti-aging function mainly due to anti-oxidation effect with little immunological effect. KEY WORDS: Epimedium acuminatum ; antioxidant-activity; aging; herbal extract 基于抗氧化作用的淫羊藿抗衰老药效研究  1 1,2 1,2 1,2 1,2* 1* 马山 ，麻培培 ，谌文元 ，陈晓 ，杜丽娜 ，田景振 (1. 山东中医药大学药学院，济南 250355 ；2.军事医学科 学院放射与辐射医学研究所，北京 100850) 摘要：目的 阐明淫羊藿抗衰老作用机制来自于其抗氧化功能而不是免疫作用。方法 皮下注射D-半乳糖建立小鼠衰老 模型，灌胃给予淫羊藿乙醇提取物。采用 ELISA 试剂盒测定丙二醛(MDA) 、超氧化物歧化酶(SOD) 、蛋白质羰基(PCO)