芝芪菌质发酵配方的筛选及主要有效成分的含量测定陈玉胜.pdfVIP

, Journal of Anhui Agri.Sci.2008, 36(4):1310-1311,1313　　　　　　　　　　　　　　　　　刘月娟　　马君叶 “” 1 1 1 ＊ 1 2 陈玉胜 ,阮鸣 ,张李阳 ,饶玉鹏 ,孙明丽 　 (1., 211171;2., 210029) 　[目的] 对芝芪菌质的不同发酵配方进行筛选, 并 对芝芪菌质中总皂苷和粗多糖含量进行测定, 从而为制定质量标准做前期准 备。[方法]芝芪菌质的水提醇沉的浓缩液用正 丁醇萃取出总皂苷;滤渣用于测定粗多糖;依照05 版药典规定用紫外分光光度法测定芝 芪菌质中总皂苷和粗多糖的含量。[结果] 采用紫外分光光度法测定芝芪菌质中总皂苷和粗多糖的平均含量, 分别为0.243 3%和 1.1616%。[结论]含2.5%黄芪药材的芝芪菌质效果更优。 　总皂苷;粗多糖;含量测定;发酵;芝芪菌质 　Q946　　　A　　　0517-6611(2008)04-01310-02 Study on the Screening of Fermentation Formula for Zhiqi Fungal Substance and Its Content Determination of Main Effective Components CHEN u-sheng et al　(Department of Life Science, Xiaozhuang ollege, Nanjing, Jiangsu 211171) Abstract　[Objective] The research aimedto screen different fermentation formula for Zhiqi fungal substance and determine the contents of total saponin and crude polysaccharide in order to makeprophasepreparationsfor establishing quality standard.[Method] Total saponinwas extractedbyN-butanolfrom concentrated solution of Zhiqi fungal substance after water extraction and alcohol sedimentation.Filter residue was used to determine the content of crude polysaccharide.According to the regulation in the pharmacopoeia (2005 edition), the contentsof total saponin crude polysaccharide inZhiqi fungal sub- stance were determined by using ultraviolet spectrophotometry .[Result] The average contents of total saponin and crude polysaccharide in Zhiqi fungal substancewere determined by using ultraviolet spectrophotometry, being 0.243 3%and 1.161 6%resp.[ onclusion] The effect of Zhiqi fungal sub- stance contained 2.5%medicinal materials Huangqi was better. Key words　Total saponin;rude polysaccharide;ontent determination;Fermentation;Zhiqi fungal substance [1-2] [7] 　　 , 、、 。, ———, 。 (Ganoderma lucidum ), ,、、、 (Astragalus membranaceus ), 、、、 (),, -、、、 O