阿奇霉素对阿霉素肾病大鼠基因表达谱的影响.pdf

现代药物与临床 Drugs Clinic 第 30 卷 第 10 期 2015 年 10 月 ·1185 · 阿奇霉素对阿霉素肾病大鼠基因表达谱的影响 郁晓腾 1, 2，张碧丽2* 1. 天津医科大学研究生院，天津 300070 2. 天津市儿童医院 肾内科，天津 300074 摘 要：目的 利用基因芯片技术了解阿霉素肾病大鼠基因表达谱及其经阿奇霉素干预后的变化。方法 将 81 只大鼠随机 分为对照组、模型组、阿奇霉素组，通过尾静脉注射法建立阿霉素肾病模型，检测第 4 、8 周时大鼠血生化指标及 24 h 尿蛋 白变化。利用Agilent 公司生产的 Agilent SurePrint G3 Rat Gene Expression （8 ×60 k ）基因芯片检测第8 周时各组大鼠的肾 脏基因表达谱，并利用生物信息学方法对检测结果进行分析。结果 与模型组比较，阿奇霉素组 24 h 尿蛋白、总胆固醇、 血肌酐均显著降低（P ＜0.01 ），白蛋白、总蛋白、内生肌酐清除率均明显升高（P ＜0.01 ）。通过芯片检测得到了各组大鼠的 肾脏基因表达谱。与对照组比较，模型组共有 185 个基因发生差异性表达；而与模型比较，阿奇霉素组共有 824 个基因发生 差异性表达。结论 阿霉素肾病的发生涉及众多基因的改变，阿奇霉素干预治疗可以改变其基因表达谱及在基因水平上使其 肾组织异常的基因表达实现回归，对进一步探索阿奇霉素干预阿霉素肾病的作用机制提供了线索。 关键词：阿奇霉素；阿霉素肾病；基因芯片；基因表达谱 中图分类号：R966 ；R983 文献标志码：A 文章编号：1674 - 5515(2015)10 - 1185 - 07 DOI:10.7501/j.issn.1674-5515.2015.10.002 Effects of azithromycin on gene expression profile in adriamycin nephropathy rats YU Xiao-teng1, 2, ZHANG Bi-li2 1. Graduate School of Tianjin Medical University, Tianjin 300070, China 2. Department of Nephrology, Tianjin Childrens Hospital, Tianjin 300074, China Abstract: Objective To study the gene expression profile of adriamycin nephropathy rats and the changes after the intervention of azithromycin by gene chip technology. Methods Eighty-eight rats were randomly divided into control group, model group, and azithromycin group. The model was established by tail vein injection of adriamycin, and the changes of blood biochemical and 24 h urine protein in rats at 4 th and 8 th week were detected. Agilent SurePrint G3 Rat Gene Expression (8×60k) gene chip were used to detect the gene expression profile, and the results were analyzed by bioinformatics method. Results Eighty-eight rats were randomly divided into control group, model group, and azithromycin group. The model was established by tail vein injection of adriamycin, and the