Pyrrosia petiolosa内生细菌筛选及其抑菌活性物质分析 - 中国测试.PDFVIP

第42卷第7期 中国测试 Vol.42 No.7 2016年7月 CHINA MEASUREMENT TEST July，2016 doi院10.11857/j.issn.1674-5124.2016.07.010 Pyrrosiapetiolosa 内生细菌筛选及其抑菌活性物质分析 杨小帆袁 宋丽菊袁 齐盼盼袁 吴道艳袁 闵 霞袁 李彩云袁 彭静珊袁赵 建 （四川大学生命科学学院资源微生物及微生物生物技术重点实验室，四川 成都 610064） 摘 要院为探究Pyrrosia petiolosa 内生细菌的抑菌活性袁采用植物组织分离和微生物传统培养法从其根尧茎尧叶共分离 检测到内生细菌14株遥通过滤纸片抑菌法筛选两株有显著抑菌活性的内生细菌G7尧Y12袁并借助GC-MS技术对这两 株内生细菌进行抑菌活性成分检测与分析遥 研究表明院 两株内生细菌经 16S rDNA 测序比对分别鉴定为 Lysinibacillus sphaericus C3-41和Bacillus amyloliquefaciens FZB42遥 生长曲线测定结果显示菌株G7尧Y12分别在 16h和12h后进入稳定期遥 GC-MS检测鉴定到两株内生细菌发酵上清抑菌活性物质主要是有机酸类和2袁5-二酮哌 嗪衍生物遥 该研究可为挖掘P. petiolosa 内生细菌药物活性尧筛选抗菌药物新来源提供参考遥 关键词院有柄石韦曰内生细菌曰抑菌活性曰GC-MS检测；代谢产物 文献标志码院A 文章编号院1674-5124渊2016冤07-0047-06 Screening of endophytic bacteria in Pyrrosia petiolosa and analysis of its antimicrobial active ingredients YANG Xiaofan，SONG Liju，QI Panpan，WU Daoyan，MIN Xia, LI Caiyun，PENG Jingshan，ZHAO Jian （Key Laboratory of Microbiological Resource and Technology，College of Life Sciences，Sichuan University， Chengdu 610064，China） Abstract: In order to study antimicrobial activity of endophytic bacteria in Pyrrosia petiolosa， plant tissue separation and traditional microbial culture methods were employed，by which fourteen bacteria strains were isolated from the roots，stems and leaves of P. petiolosa. Endophytic bacteria G7 and Y12 were screened out due to their significant antimicrobial activity through filter paper bacteriostatic method. The antimicrobial active ingredients were detected and analyzed by GC-MS technology. Strains G7 and Y12 were identified as Lysinibacillus sphaericus C3-41 and Bacillus amyloliquefaciens FZB42 respe