果蔬总抗氧化能力间接测定法及其影响因素-食品科学.PDF

※专题论述 食品科学 2008, Vol. 29, No. 04 457 果蔬总抗氧化能力间接测定法及其影响因素 付陈梅1,3，焦必宁1,2,3,*，阚建全1 (1.西南大学食品学院，重庆 400716；2.中国农业科学院柑桔研究所，重庆 400712； 3.农业部柑桔及苗木质量监督检验测试中心，重庆 400712) 摘 要：果蔬抗氧化能力是衡量果蔬抗氧化成分作用强弱的主要参数，评价总抗氧化能力的方法有直接法和间接 法，其中，间接法是最为快捷、简便的分析方法。ABTS 法和DPPH 法是目前应用最为普遍的间接测定方法；间 接测定的影响因素有底物及其相互之间的作用、体系中的干扰物质、pH 值、反应时间、自由基产生体系等。研 究人员试图用 HPLC 等方法与间接法联用以减少外界因素对检测结果的影响。 关键词： 间接测定法；ABTS；DPPH ；抗氧化能力 Indirect Methods and Influence Factors to Determine Total Antioxidant Activity of Fruit and Vegetable 1,3 1,2,3,* 1 FU Chen-mei , JIAO Bi-ning , KAN Jian-quan (1.College of Food Science, Southwest University, Chongqing 400716, China； 2.Citrus Research Institute, Chinese Academy of Agricultural Sciences, Chongqing 400712, China；3. Unality Supervision, Inspection and Testing Center for Citrus and Seadings, Mininstry of Agriculture, Chongqing 400712, China) Abstract: The total activity is the main parameter to value the antioxidant ability of objects. Methods to measure total antioxidant activity can be divided into indirect and direct methods according to priciple, and indirect methods were fast and simple ways to determine the total antioxidant activity. At present, ABTS and DPPH were applied widely. The results of indirect methods were time, producing system for free influenced by factors such as antioxidants and interactions, interference materials, pH, action radicals and so on. Researchers tried to use the other advanced methods such as HPLC to decrease the environmental influence on the indirect method. Key words: indirect metho