云芝（Trametes versicolor）1126漆酶粗酶液对靛蓝染料脱色的初步研究.PDF

Mycosystema 菌 物 学 报 15 March 2008, 27(2): 309-315 jwxt@ ISSN1672-6472 CN11-5180Q ©2008 Institute of Microbiology, CAS, all rights reserved. 云芝 Trametes versicolor1126所产漆酶对靛蓝废水 脱色的初步研究 1 1,2 1,2 1 1∗ 闫世梁 李培睿 李宗义 李宗伟 秦广雍 1 郑州大学离子束生物工程省重点实验室 郑州 450052 2 河南师范大学生命科学学院 新乡 453007 摘 要：考察了云芝Trametes versicolor 1126 发酵培养中漆酶酶活和pH 的变化，同时研究了羧甲基纤维 素钠及苯酚添加量对漆酶活力的影响。结果表明当培养基中加入0.8%羧甲基纤维素钠、100mg/L 苯酚时， 均能明显提高漆酶的活力。以漆酶/HBT 介质体系对靛蓝废水进行脱色，反应100min 后，脱色率达90.8% 。 使用漆酶处理靛蓝废水具有广阔的应用前景。 关键词：大型真菌，纺织染料，降解 Preliminary study on decolorization of indigo dye wastewater with laccase from Trametes versicolor 1126 YAN Shi-Liang1 LI Pei-Rui1, 2 LI Zong-Yi1, 2 LI Zong-Wei1 QIN Guang-Yong1* 1Provincial Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, China 2 College of Life Sciences, Henan Normal University, Xinxiang 453007, China Abstract: The dynamic variation of laccase activity and pH were detected during fermentation of Trametes versicolor 1126. The effects of sodium carboxymethyl cellulose and phenol on laccase activity were also studied. The results indicated that 0.8% sodium carboxymethyl cellulose and 100mg/L phenol in the medium can enhance the activity of laccase obviously. Indigo dye wastewater was degraded in laccase/HBT mediator system. The decolorization rate reaches 90.8% in 100 minutes. A practical way using laccase to decolorize indigo dye 基金项目：国家重点基础研究发展规划973 项目（No. 2004CB719604 ） ∗Corresponding author. E-mail: qinguangyong@ 收稿日期：2007-06-12 ，接受日期：2007-10-09 310 Mycosystema wastewater has extensive prospect. Key words: larger fungi, te