小鼠烧伤早期免疫细胞基因表达谱分析及靶基因的筛选.PDFVIP

J South Med Univ, 2017, 37(6): 767-773 doi 10.3969/j.issn. 1673-4254.2017.06.09 ·767 · 基础研究 小鼠烧伤早期免小鼠烧伤早期免疫细胞基因表达谱分析及靶基因的筛选疫细胞基因表达谱分析及靶基因的筛选 邹 琼，高艳彬，金 辉，卢志阳，史鹏伟，杨 磊 南方医科大学南方医院烧伤科，广东 广州 510515 摘要：目的利用生物信息学方法对小鼠烧伤早期免疫细胞差异基因表达谱进行分析并检测，筛选相关差异基因，以期找到潜在 的诊断及治疗靶标。方法 从GEO 数据库中下载GSE7404 数据集，通过配对样本t 检验、倍比法及GO 功能富集分析筛选差异 表达基因，应用STRING 数据库及Cytoscape 软件构建蛋白互作网络筛选相关基因靶标，运用实时荧光定量PCR 及Western blotting 进行试验验证。结果 在烧伤后第1天，有1825个基因被选出，其中上调基因658 个，下调基因1167个。运用STRING 数据库及Cytoscape 软件构建蛋白互作网络分析，发现Stat1、Cdk1、Cd19、Lck 及Jun 基因在烧伤后免疫系统功能变化过程中可能 起到重要作用。实时荧光定量PCR 及Western blotting 检测显示Stat1、Cdk1和Jun 基因表达情况与分析结果相一致。结论 通 过对小鼠烧伤后早期全血游离白细胞基因芯片分析我们发现Stat1、Cdk1 和Jun 基因在烧伤早期免疫细胞功能变化的过程中发 挥重要的作用，可能是烧伤早期诊断及治疗的潜在的生物学靶标。 关键词：烧伤；免疫系统过程；差异表达基因；蛋白互作网络 Screening of biomarkers related with leukocyte responses early after burn injury in mice by differential gene expression profiling ZOU Qiong, GAO Yanbin, JIN Hui, LU Zhiyang, SHI Pengwei, YANG Lei Department of Burns, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China Abstract: Objective To screen the genes related with leukocyte responses in mice early after burn injury by bioinformatic analysis of the gene expression profiling data. Methods The gene expression profiles were obtained from GEO (GSE7404, Mouse musculus, 25% TBSA, full-thickness) database. T test, fold changes and GO functional enrichment analysis were used to identify the differentially expressed genes (DEGs) related to leukocyte responses to burns; the interacting genes were transferred to STRING to construct the protein-protein interaction (PPI) network. Biological annotation of the sub-networks was executed using the software Cytoscape. Real-time PCR and Western blotting were used to verify the DEGs in mice. Results In mice at 1 day post-burn, a total of 658 genes were up-regulated and 1