P- 糖蛋白表达与原发性肝癌合并门静脉.PDFVIP

· 3 5 0 · 中华普通外科学文献（电子版）2013 年 10 月第 7 卷第 5 期 Chin Arch Gen Surg（Electronic Edition)，October 2013，Vol.7，No.5 ·论著 · P- 糖蛋 白表达与原发性肝癌合并 门静脉 癌栓患者术后辅助化疗效果 的相关性研究 胡文杰　黎东明　陈伟　张昆松　黄力　梁力建 　　【摘要】　目的　研究 P- 糖蛋 白 （P-gp ）表达与原发性肝细胞癌合并 门静脉癌栓患者表达 情况与临床化疗效果之间的关系。方法　收集术后接受化疗 的 29 例原发性肝细胞癌合并 门静脉癌 栓患者的石蜡块 ，构建组织芯片。采用免疫组化染色技术 ，检测 P-gp 蛋 白的表达情况 。根据 P-gp 蛋 白表达强弱分为耐药组和非耐药组 ，进行生存分析 。结果　P-gp 蛋 白表达 的阳性率为 75.86 ％ （22/29 ）。中位无瘤生存期在耐药组 （20 例 ）与非耐药组 （9 例 ）间有显著差别 。多因素 Cox 回 归分析显示 P-gp 表达情况为无瘤生存期 的独立影响因子 。结论　P-gp 的强表达是导致肝癌耐药 、 化疗效果欠佳 的原 因之一 ，P-gp 有可能作为临床筛选化疗患者的指标 。 　　 【关键词】　P- 糖蛋 白；肝细胞癌 ；癌栓 ；门静脉 ；化疗 Correlation between the expression of P-gp and efficacy of chemotherapy postoperation in hepatocellular carcinoma patients with tumor thrombi in major portal vein HU Wen-jie, LI Dong- Ming, CHEN Wei, ZHANG Kun-song, HUANG Li, LIANG Li-jian. Department of Hepatobilliary Surgery, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China Corresponding author: LIANG Li-jian, Email:lianglj@ 　　 　To investigate the expression of P-glycoprotein (P-gp) and its correlation 【Abstract 】　Objective to chemotherapeutic efficacy in hepatocellular carcinoma (HCC) with tumor thrombi in major portal vein. Methods　Twenty-nine patients of HCC with tumor thrombi in major portal vein following hepatectomy and thrombectomy were enrolled in this study, all the patients received immunochemotherapy postperatively. Specimens embedded in paraffin were collected for the construction of tissue microarrays. P-gp protein were visualized by immunohistochemical staining using Monoclonal antibodies JSB-1. The patients were divided into chemotherapy sensitive and resistant groups according to the staining grade. The intensity ≥ (++) were considered resistant to chemotherapy. Survival time was