单纯激素-皮下球囊-A rat model of early stage osteonecrosis induced by glucocorticoids.pdfVIP

Kerachian et al. Journal of Orthopaedic Surgery and Research 2011, 6:62 /content/6/1/62 RESEARCH ARTICLE Open Access A rat model of early stage osteonecrosis induced by glucocorticoids 1,2 3 1,4,5 5 6 Mohammad Amin Kerachian , Edward J Harvey , Denis Cournoyer , Terry Y Chow , Ayoub Nahal and Chantal Séguin4,5* Abstract Background: Glucocorticoid (GC)-induced osteonecrosis (ON) is an important complication of medical therapy. The exact pathomechanisms of ON has not been clearly elucidated. There is a need for a reproducible animal model that better approximates the clinical scenario. Methods: To determine the genetic susceptibility of rats to develop GC-induced femoral head ON, we evaluated 5 different inbred strains of rats (Spontaneous Hypertensive Rat, Wistar Kyoto, Wistar Furth, SASCO Fisher and Lewis). Prednisone pellets (dosage of 1.82-2.56 mg/kg/day) were implanted subcutaneously for 90. After 90 days, the femurs were resected and examined histologically and radiographically. Pathological and histological examination was performed. Hematoxylin and eosin (H E) staining was used to delineate the femoral head osteonecrosis lesions as well as abnormalities of articular cartilage and growth plate. Results: The greatest differences in H E staining were seen in the Wistar Kyoto and Wistar Furth groups. In these groups 4 out of 5 and 3 out of 5, respectively, steroid-induced rats revealed growth plate disruption with acellular areas. The TUNEL apoptosis staining assay for apoptosis revealed that 4 out of 5 of Wistar Kyoto rats, 5 out of 5 of Wistar Furth, 2 out of 4 of surviving Lewis and 2 out of 2 of the surviving spontaneous hypertensive rats had apoptotic o