骨折愈合的试验研究.PDFVIP

98 CHINESEjoURNALOFOSTEOPOROSISVo1．1NO．21995 BMP促进去神经后废用性骨质疏松 骨折愈合的实验研究 徐栋梁 季佛保 于毅力 丘钜世 摘要 为了观察BMP对去神经后废用性骨质疏松性骨折愈合的作用．作者选用6月龄健康标准 太鼠，严格配对分组，切除双侧坐骨神经后3周证实有明显骨质疏松。然后在胫骨人工骨折骨缺损处分 别植入酸解腔状鼠尾胶原(对照)厦BMP与腔原混和物(实验组)。通过血清钙浓度、碱性磷酸酵梧性厦 骨组织形态计量学多项参数的观察．显示BMP对去神经后废用性骨质疏松骨折禽台有明显促进作用 fP0．05～0．01) 关■调 骨形态形成蛋白 废用性骨质疏松症 骨折愈合 Experimentalstudyabouttheeffectsofbonemorphogetteticprotein 0nfracturehe~lngofpost-deRervedisusedosteoporosis XuDongliang，LiFohaotYuYili，etal (DepartmentofOsteopaeticstheFirstAffiliatedHospi~l，and PathologytSunYet—senUniversityofMedicalSciences) AbstractInordertOstudytheeffectsofbonemorphogeneticprotein(BMP)onthefracturehealing ofpost，denervedisusedosteoporosJs，20six—montholdSD Ratshadbeenchosenforsciaticneuzeeto— my．Thentheratmodelofdisusedosteoporosiswereestablishedafter3weeks．3ram dbiadraftdefects werecreatedbetweenSegmentsofupper1／3andmiddle1／3．Defectsof10ratswereimplantedwithdi— gestedcoloidabstractedfrom collagenofrattailsascontro1．Theother10ratswereimplantedwithmix- tureofthecoloidandBMPasexperimentalgroup．Throughtheinvestigationonseraconcentrationof calcium 。activityofseraalkalinephosphatase，andsomeindicesofbonehistomorphometryintheregion ofcalllUStwhichincludedtrabeeularvolums．osteoidvolrimsosteoidsurfacesosteoblastiesurface$tmln- erallzationrates，thisstudydemonstratedthatBMPcouldacceleratedthebonefracturehealinginpost— denervedisusedosteoporosisofrats． Keywords Bonemorphogenetieprotein Disusedosteoporosis Bo nefractruehealing 支配某段躯干和肢体的神经完全损伤后． 直接植入该种骨质疏松骨折骨缺损处，观察 该神经所属骨骼肌逐渐出现萎缩瘫痪，相应骨 BMP的成骨诱导作用。 出现废用性骨质疏松症；神经破坏后亦可直接 1 材料和方法 引起骨代谢降低，骨质形成过程缓慢或停顿，进 选用6月龄健康SD大白鼠，雄性，共 20 一 步促进骨质疏松化，这种情况下并发及继发