韶关淮山多酚氧化酶酶学特性研究 - 广东农业科学.pdfVIP

东农业科学 2012 年第 4 期 81 韶关淮山多酚氧化酶酶学特性研究 廖明星 朱定和 罗杰宇 渊韶关学院英东食品科学与工程学院 广东 韶关 512005 冤 摘要 采用分光光度法研究了韶关本地淮山多酚氧化酶的活性 分析了 pH 值尧温度和抑制剂对淮山多酚氧化酶活性的影 响遥 结果表明 淮山多酚氧化酶的最适 pH 值为 6.0 最适温度为40益曰NaCl尧柠檬酸尧L-抗坏血酸对多酚氧化酶均有抑制作用遥 关键词 韶关淮山曰 多酚氧化酶曰 酶学特性曰 抑制剂 + 中图分类号 TS201.2 5 文献标识码 A 文章编号 1004-874X渊2012冤04-0081-03 Study on enzymology properties of polyphenol oxidase in Shaoguan Yam (Dioscorea fordii Prain et Burkill) LIAO Ming-x ing, ZHU Ding-he, LUO J ie-yu (College of Y ingdong Food Science Tec hnology, Shaoguan Univ ersity, Shaoguan 512005, China) Abstract: The enzymology properties were studied on polyphenol oxidase in Saoguan Yam (Dioscorea fordii Prain et Burkill) by means of spectrophoto -metrically determining the activity at 410 nm with catechol as substrate, on the conditions of different pH, temperature and inhibitor concentration援The results showed that the optimum conditions were pH6.0 and temperature 40益, and that L- ascorbic acid, critic acid and NaCl all lowered the enzyme activity by inhibited the catalysis. Key words: Shaoguan Yam (Dioscorea fordii Prain et Burkill); polyphenol oxidase; enzymogy properties; inhibitor 淮山渊Dioscorea Oppos ita Thunb.冤 又称薯蓣渊Dioscorea 不是河南栽培的怀山药 而是当地野生山薯长期家化栽培 batatas Decne冤 别名山药 是我国特有的物种 为百合目 [9] 而形成的 遥 在质量方面 与药典收载的山药渊原植物为薯 渊Liliales冤薯蓣科渊Dioscorea 冤薯蓣属渊Dioscorea L.冤 是一年 蓣 D. oppos ite Thunb.冤比较 其有效成分如多糖和氨基酸 [1]