东亚砂藓类萌发素蛋白基因rjglp 的克隆及表达分析 - 湖南农业大学 .pdfVIP

() 2015 2 41 1 Journal of Hunan Agricultural University (Natural Sciences), Feb. 2015, 41(1):047–052 DOI:10.13331/ki.jhau.2015.01.009 东亚砂藓类萌发素蛋白基因 RjGLP 的克隆及表达分析 1 1,2* 2 2 (1. 150040 2. 161006) 摘 要PCR cDNA RjbZIP 726 bp669 bp 222 23 397.9(pI ) 6.8214.41 GLPs PCR RjGLP (CK) 关 键 词RjGLP 中图分类号Q785 文献标志码：A 文章编号：1007− 1032(2015)01−0047−06 Cloning and expression analysis on RjGLP, a germin-like protein gene from Racomitrium japonicum 1 1,2* 2 2 Zhang Meijuan , Sha Wei , Liu Bo , Xu Honghong (1. College of Life Sciences, Northeast Forestry University, Harbin, Heilongjiang, 150040, China; 2. College of Life Science and Agriculture and Forestry, Qiqihar University, QiqiharHeilongjiang 161006, China ) Abstract: A full length cDNA sequence of germin-like protein was cloned from Racomitrium japonicum using RT–PCR, and was named as RjGLP, which was 726 bp in length, contained an open reading frame (ORF) of 669 bp, and encoded with 222 amino acids. The results of bioinformatics demonstrated that the protein belonged to stable protein with molecular weight of 23 397.9 and wtih pI of 6.82. The sequence prediction showed that the protein, located in cytoplasm, was a transmembrane protein and had signal peptide. Multiple sequence alignment of GLP proteins revealed that the protein had typical characteristics of plant germin-like proteins. The RT–qPCR results showed that RjGLP gene could express in the process of quick dehydration, and the expression was higher than that of CK. Therefore, the RjGLP gene was speculated