葛根素对大鼠局灶性脑缺血再灌注后神经细胞凋亡及Bcl_2_Bax蛋白表达的影响.pdf

2008 10 6 10 # 117 # Bc-l 2Bax 1) 韩江全, 李官成, 周晓兰, 朱选平, 梁 恒, 李继中, 林冬融, 赖 敏 : 探讨葛根素对大鼠局灶性脑缺血再灌注损伤的保护作用 制作大鼠大脑中动脉缺血再灌注模型40 只 SD 性大鼠被随机分为假手术组缺血组小剂量葛根素治疗组( 小剂量组) 大剂量葛根素治疗组( 大剂量组) 应用 TT C 染色观 察梗死体积, 应用免疫组化染色及TUNEL 染色检测Bcl-2Bax 蛋白表达及凋亡细胞数 大小剂量组梗死体积和凋亡细胞 数明显少于缺血组(P 0.01) , Bc-l 2 明显多于缺血组(P 0. 01) , 小剂量组Bax 少于缺血组( P 0. 05) , 大剂量组Bax 明显少于缺血 组(P 0.01) 大剂量组梗死体积和凋亡细胞数少于小剂量组(P 0. 05) , Bc-l 2 多于小剂量组( P 0. 05) 大小剂量组 Bax 无统 计学意义(P 0.05) 葛根素可通过上调Bc-l 2 蛋白表达, 下调Bax 蛋白表达减少脑缺血再灌注后神经细胞凋亡, 疗效与剂 量有关 : 葛根素; 脑缺血再灌注; 凋亡 : R743. 31 R28 . 5 : A : 1672- 134 ( 2008) 10- 117 -03 The Effect of Puerarin on Cell Apoptosis and Expression of Bc-l 2 and Bax Protein in Focal Cerebral Ischemia-Reperfusion Han Jiang uan, Li Guancheng, Zhou Xiaolan,et a l Depart ment of Neurology , The 5th Affiliat ed H ospital, Zunyi Medical College(Zhuhai 51 100) Abstract:Objective To study the effect of puerarin on the expression of Bcl-2 and Bax protein in focal cerebral ischemia-reperfu- sion. Methods The rat models w ere established by middle cerebral artery occlusion ( MCAO) and reperfusion. T otal of 40 healthy male Sprague Daw ley ( SD) rates were divided randomly into 4 groups: Sham operation group, cerebral ischemia group, large dose of puerarin group and low dose of puerarin group. T he ischemic zone w as detected by tetraphenyl tetrzolium chloride staining . The ex- pression of Bc-l 2 and Bax protein w ere analyzed by immunohistochemistry. The neural cells apoptosis were measured by terminal de- oxynucleotidyl transferase-mdiated dUTP nick end labeling (T UNEL) . Results Compared w it h the cerebral ischemia group, the size