绿色木霉产双功能酶二级结构的ft-ir光谱法分析.pdfVIP

150 2012, Vol. 33, No. 17 食品科学 ※生物工程 FT-IR 1 1 1 2, 刘 萍 ，刘 靖 ，祁兴普 ，夏文水 * (1.江苏畜牧兽医职业技术学院食品科技学院，江苏省畜产品深加工工程技术研究开发中心，江苏 泰州 225300 ； 2.江南大学食品学院，食品科学与技术国家重点实验室，江苏 无锡 214122) ：采用傅里叶红外光谱(FT-IR) 的酰胺Ⅰ带和酰胺Ⅲ带测定绿色木霉产双功能壳聚糖酶- 纤维素酶(CCBEs) 的 天然二级构象，结果显示：FT-IR 光谱酰胺Ⅰ和Ⅲ带可以用来对绿色木霉所产的双功能酶二级结构进行预测，对 两种双功能酶的主要二级结构组分 α- 螺旋和 β- 折叠的测定误差分别在2% 和4% 范围内，CCBE Ⅰ的二级构象以β- 折叠为主，高达 70% ，与其圆二色谱和Garnier-Robson 预测结果基本一致；而 CCBE Ⅱ的二级构象中的β- 折叠和 α- 螺旋含量分别为 53.6% 和 14.6%。 ：F T - I R 光谱；二级结构预测；壳聚糖酶；纤维素酶；绿色木霉 Secondary Structure of Bifunctional Chitosanase-Cellulase from Trichoderma viride as Determined by FT-IR Spectrometry 1 1 1 2, LIU Ping ， LIU Jing ，QI Xing-pu ，XIA Wen-shui * (1. Jiangsu Engineering Research and Development Center for Livestock Product Processing Technology, School of Food Science and Technology, Jiangsu Animal Husbandry and Veterinary College, Taizhou 225300, China ；2. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China) Abstract ：The secondary structure of two bifunctional chitosanase-cellulase enzymes (CCBE Ⅰ and CCBE Ⅱ) from Trichoderma viride was explored by FT-IR spectroscopy. Amide Ⅰ and Ⅲ FT-IR bands allowed prediction of the secondary structure of CCBE. The errors of determination for α-helix and β-sheet contents in CCBEs were less than 2% and 4%, respectively. The quantitative analysis of CCBE Ⅰ revealed that the amino acid sequence was con