甘蓝型油菜表皮特异硫蛋白(esp)的序列分析及其诱导表达 - 食品科学.pdfVIP

※生物工程 食品科学 2012, Vol. 33, No. 07 209 (ESP) 王 政，谭小力，张志燕，顾守来，李冠英 (江苏大学生命科学研究院，江苏 镇江 212013) 为了揭示植物激素及环境胁迫对甘蓝型油菜(Brassica napus)表皮特异硫蛋白(ESP)表达的影响，以中双 9 号 品种为材料，采用同源克隆法获得一编码甘蓝型油菜表皮特异硫蛋白(BnESP) 的cDNA ，序列分析表明其编码蛋白 BnESP 含有 343 个氨基酸，与青花菜(Brassica napus)ESP 有 99% 的相似性，与拟南芥腈特异性蛋白、类黑芥子酶 结合蛋白、茉莉酸诱导蛋白有较高的同源性。荧光定量RT-PCR 分析显示，甲基茉莉酸和机械伤害快速激活BnESP 的表达，苯丙噻重氮和核盘菌(Sclerotinia sclerotiorum)抑制其表达。研究证明 BnESP 是茉莉酸诱导蛋白，可能在 水杨酸和茉莉酸信号的互作及油菜对S. sclerotiorum 的反应网络中起作用。 油菜；表皮特异硫蛋白；基因克隆；诱导表达 Sequence Analysis and Induced Expression of Epithiospecific Protein (ESP) from Brassica napus WANG Zheng ，TAN Xiao-li ，ZHANG Zhi-yan ，GU Shou-lai ，LI Guan-ying (Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China) Abstract：In order to explore the effect of plant hormones and environment stress on the expression of Brassica napus ESP (BnESP), a cDNA encoding BnESP was cloned from Zhongshuang No. 9 by homology cloning approach. Sequence analysis showed that BnESP had 343 amino acids, and revealed 99% identity with brassica oleracea ESP and a high homology with nitrile- specific proteins (NSP), MY-binding-like protein (MBP) and JA inducible protein (JIP). Quantitative RT-PCR analysis showed that the expression of BnESP were promptly activated by MeJA and by wound treatment, and suppressed by Benzothiadiazole and by Sclerotinia sclerotiorum. This study suggests that ESP is a JIP and plays an important role in crossing-talk between SA and JA signaling and in the molecular network of oilseed rape response against S. sclerotiorum. Meanwhile, this study can provide a cue for producing health foods through attenuating the expression of ESP in cruciferous plants. Key words ：oilseed rape ；epit