白桦APETALA2（AP2）转录因子基因的分离及其表达.pdfVIP

林业科学研 究 2012，25(2)：254～260 ForestResearch ． ． ． ． ． ． ． ．． ． ． ． ． 。 ． ． ． ． ． ． ． ． ． ． ． ． ． — — 文章编号：1001—1498(2012)02-0254-07 白桦 APETALA2【AP2)转录因子基因的 分离及其表达 张 妍，刘 瀛，孙丰宾，戴 超 ，刘雪梅 (东北林业大学生命科学学院，黑龙江 哈尔滨 150040) 关键词：白桦；AP2；转录表达；qRT—PCR；花发育 中图分类号：$718．46 文献标识码 ：A Isolation andExpression ofAPETALA2Transcription FactorGeneinBetulaplatyphy ZHANG Yah，LIU Ying，SUN Feng—bin，DAIChao，LIUXue—mei (CollegeofLifeScience，NortheastForestlTUniversity，Harbin 150040，Heilongjiang，China) Abstract：AP2geneisakeytranscriptionfactorinvolvedinflowerdevelopmentinplants．ThefulleDNA ofAP2 genewasisolatedfromBetulaplatyphyllaSuk．bymethodsofreversetranscriptionpolymerasechainreaction(RT— PCR)and5’and3’rapidamplificationofcDNAends(RACE)．ResultsshowedthatAP2genecontainsanopen readingrfame(ORF)of1554bpencoding517aminoacids．Molecularweightofthededucedproteinof@Am is 56．74kDaandthetheoreticalisoelectricpointis6．34．TheAP2 functionalsitesand characterized domainswere confirmedinthesequence．SOtheisolatedgenewasnamedasBpAP2，andregisteredinGenBankwith accession numberJN247408．Thededucedaminoacidsequenceshared51％～77％ ofidentitywithothertwelveplantspe— cies．themaximumidentitywithArabidopsisthaliana(77％)andndnimumidentitywithPinusthunbergii(51％)． A phylogenetictreewasconstructedaccordingtomultiplesequencesalignmentofallthethirteenplantspecies． TranscriptionexpressionofBpAP2wasanalyzedbyqRT—PCR indifferenttissuesandperiodsinB．platyphylla．Re— suhsshowedthatBpAP2wasmorehighlyexpressedinfloralorgansthan in vegetativeorgans，expression quantity morehighlYinyoungtissuesthaninmaturetissues．ItinferredthatBpAfy2transfactorinvolvedintheregulationof