量子点复合微球的活体荧光和化学发光双模式成像.pdf

论 著 RESEARCH WORK 量子点复合微球的活体荧光和化学发光 双模式成像 Quantum Dots Composite Microspheres for In Vivo Fluorescence and Chemiluminescence Dual-Mode Imaging 1 1 1 [摘 要] 为了探索荧光/化学发光双模式成像方法用于可降解生物材料研究的可行性，将载有量子 叶一 ，王国武 ，王莉萍 ， 和田智之2 1 点的可降解高分子微球（量子点复合微球）植入小鼠背部皮下，利用美国Caliper定量荧光和生物 ，王瑾晔 1.上海交通大学 生物医学工程学院， 发光成像系统IVIS Lumina II观察不同时间点植入部位的荧光强度和L-012介导的化学发光强度的 上海 200240 ；2. 日本理化学研究所先 变化。结果显示：30 mg量子点复合微球植入28 d时，仍能检测到荧光信号。L-012介导的化学发 进光子学中心，和光埼玉 351-0198 光强度随时间逐渐减弱，第28 d时，量子点复合微球处仍存在微弱的化学发光。 [关键词] 量子点；复合微球；活体荧光成像；活性氧；化学发光 Abstract: To develop in vivo fluorescence and chemiluminescence dual mode imaging method for materials, quantum dots (QDs) loaded with biodegradable polymer microspheres (BPM) were subcutaneously injected into the back of mice. L-012 was employed to noninvasively monitor the product of reactive oxygen species. The fluorescence intensity and chemiluminescence intensity were observed by Quantitative Fluorescent and Bioluminescent Imaging System IVIS Lumina II from Caliper at different time points. The results showed that the fluorescence of 30 mg BPM/QD could be detected up to 28 d. 1 1 Meanwhile, chemiluminescence intensity of the implanted microspheres induced by L-012 decreased YE Yi , WANG Guo-wu , WANG Li-ping1 2 with time. On day 28, there was still weak chemiluminescence where the microspheres were implanted. , WADA Satoshi , WANG Jin-ye1 Key words: quantum dots; composite microspheres; in vivo fluorescence imaging; reactive oxygen 1.School of Biomedical Engineering, species; chemiluminescence Shanghai Jiao Tong University, Shanghai 200240, China;