超滤—离子层析法精制猪硫酸软骨素-吉林农业大学学报.PDF

吉林农业大学学报 2012 ，03 Journal of Jilin Agricultural University E-mail:jlndxb@ 超滤—离子层析法精制猪硫酸软骨素* 1 1 2 3 1 1 1** 陆 钊 ,董树国 ,王 玥 ,曹宏梅 ,赖红伟 ,康永炜 ,董顺福 1.吉林医药学院药学院，吉林 132013 ； 2. 吉林石化分公司电石厂，吉林 132022 ； 3. 吉林医药学院实 验中心基础化学实验室，吉林 132013 摘 要:采用超滤-离子层析法精制猪硫酸软骨素，离子对反相高效液相色谱法测定其含量，红外吸收光谱法测定其 结构。研究了超滤液温度、超滤压力、超滤液pH对猪硫酸软骨素精制时膜通量和得率的影响。结果表明：超滤— 离 子层析技术可应用于精制猪硫酸软骨素。猪硫酸软骨素超滤的工艺条件：以微孔滤膜处理原料液，超滤膜的截留分 子量10 kD，超滤压力0.25 MPa ，超滤温度25 ℃，溶液pH 为9 。产物纯度为95.74%,产率为80.15% 。该方法精确度和 准确度都较高，可用于猪硫酸软骨素的精制。 关键词:猪硫酸软骨素；超滤膜法；离子交换层析法；精制 中图分类号：R282.74 ；S828        文献标识码：A 文章编号：1000-5684(2012)  DOI: 网络出版地址： /kcms/detail/22.1100.S1433.001.html Refinement of Porcine Chondroitin Sulfate by UF Membrane-Ion- Exchange Chromatography 1 1 2 3 1 1 LU Zhao ,DONG Shu-guo ,WANG Yue ,CAO Hong-mei ,LAI Hong-wei ,KANG Yong-wei , DONG Shun-fu1 1. College of Pharmacy, Jilin Medical College , Jilin 132013,China; 2. Calcium Carbide Factory of Petro China，Jilin Petrochemical Co. LL.td. ，Jilin 132022，China; 3..Basic Chemistry Laboratory of Experimental Center, Jilin Medical College , Jilin 132013,China Abstract ：Porcine chondroitin sulfate was refined by UF membrane- ion-exchange –chromatography.Its structure was determined with infrared spectroscopy and its content was determined by Ion-pair RP—HPLC. The effects of ultrafiltrate temperature, ultrafiltration pressure and ultrafiltrate pH on the flux and yield of porcine chondroitin sulfate were studied when it was refined. UF membrane-ion-exchange chromatography can be used to refine porcine chondroitin sulfate. The process conditions of the refinement of porcine chondroitin sulfate: using microporous membrane to proce