马铃薯渣果胶多糖分离纯化及结构初探.pdfVIP

安徽农业科学,Journal of Anhui Agri .Sci .2008 ,36( 19) :7970 - 7973 责任编辑 姜丽 责任校对 卢瑶 马铃薯渣果胶多糖分离纯化及结构初探 * 万建华, 顾正彪 , 洪雁 ( 江南大学食品学院食品科学与技术国家重点实验室, 江苏无锡214122) 摘要 [ 目的] 初步探 索马铃薯渣果胶多糖 的一级结构 。[ 方法] 采 用阴离子交换柱分 离, 交联 葡聚糖凝胶柱纯化, 气相 色谱, 高效凝胶 色 谱, 紫外扫描和红外光谱 、高碘 酸氧化和Smith 降解等方法, 对马铃薯渣果胶多糖 的一 级结构进行 了初步推测。[ 结果] 马铃薯渣 果胶多 糖 由酸性糖和 中性糖组成, 其中大部分为酸性糖, 酸性糖分子量为41 161 Da , 其 中不含蛋 白、肽链和核酸, 既有五碳糖也 有六碳糖, 既有 吡糖又有呋糖, 其结构分枝很少, 既有 α- 糖苷键 又有 β- 糖苷键, 戊糖是 以1→2 或1 →4 位键合 的, 己糖则是 以1 →4 或1→3 位键合的。 [ 结论] 马铃薯渣果胶多糖是一种结构复杂的以糖醛酸为主的酸性杂多糖 。 关键词 马铃薯渣果胶; 分 离纯化; 结构 中图分类号 S532 文献标识码 A 文章编号 0517 - 66 11( 2008) 19 - 07970 - 04 Studies on Isolation and Purification and Structures of Polysaccharide in Potato Pulp Pectin WAN Jian- hua et al ( School of Food Science and Technology , Jiangnan University , Wuxi , Jiangsu 214122) Abstract [ Obj ective] The research ai medto study the structure of polysaccharides in potato pul p pectin . [ Method] By using anion- exchange column for isolation and sephadex column for purification , GC , HPGPC , ultraviolet scanning and infrared spectrum, periodate oxidation and Smith degradation method and so on , the structure of polysaccharides in potato pul p pectin were eval uated . [ Result] Polysaccharides in potato pulp pectin were co mposed of most acidic polysaccharide and a little neutral polysaccharide . The molecular weight of acidic polysaccharide was 41 161 Da and di d not contain proteins , pep- tide chains and nucleic acid . But it contained both pentoses and hexoses , both pyranoses and furanoses , less branch structure , both α- glycosidic linkage and β- glycosidic li nkage . The pentoses were bonded by 1 →2 or 1 →4 site and hexoses by 1 →4 or 1 →3 site . [ Conclusion] Polysaccharide in potato pul p pectin was a kind of acidic polysaccharide with co mplex structure containing much alduronic acid . Key word