LC-MS／MS联用技术分析野马追及复方野马追胶囊中的黄酮类成分.pdfVIP

147 LC—MS／MS联用技术分析野马追及复方野马追胶囊 中的黄酮类成分 m 张晓玲 ，段金廒 ，钱大玮 ( 南京医科大学公共卫生学院，南京210029； 南京中医药大学江苏省方剂研究重点实验室，南京210029) 摘 要 目的：分析野马追及野马追胶囊中的黄酮类成分。方法：采用Phenomenex—C 柱(250 mm×4．6 mm，5 txm)， 流动相：乙腈(A)-0．4％冰醋酸溶液，梯度洗脱，0 min(5％A)一60 min(95％ A)；流速：1．0 mL／min；检测波长：280 nm；运 用LC—MS／MS对野马及野马追胶囊中的黄酮类成分进行分析。结果：共分析了6个黄酮类成分，确定了3个黄酮苷：金丝桃 中一 圄 {8 苷(hyperoside)、三叶豆苷(trifolin)和黄芪苷(astragalin)；3个黄酮苷元：槲皮素(quercetin)、山柰酚(kaempfero1)和棕矢车菊 素(jaceosidin)。结论：LC—MS／MS能快速、准确地分析并确定天然产物中的化学成分。 兽 关键词 野马追；复方野马追胶囊；黄酮；高效液相色谱一串联质谱法 种 中图分类号 R917 文献标识码 A 文章编号 1000—5048(2008)02—0147—04 夫 擎商报瑚 学y Identification of flavonoids frOm Eupatorium lindleyanum and compound Yemazhui capsules by LC—MS／MS ZHANG Xiao—ling 。DUAN Jin—ao ，QIAN Da—wei School ofPublic Health，Nanfing Medical University,Nanjing 210029； Jiangsu Key Laboratoryfor TCM Formulae Research，Nanjing University of TCM,Nanfing 210029,China Abstract Aim：To verify flavonoids fron Eupatorium lindleyanum and compound Yemazhu capsules．Meth- ods：HPLC COUpled with a quadrupole tandem mass spectrometer was applied to analyze the structures of flavonoids in Eupatorium lindleyanum and compound Yemazhui capsules．Results：Three flavonoid aglycone and three flavonoid glycosides were confirmed based on this method．Conclusion：This method iS helpful for the rapid discovery and identification of flavonoids from the natural products． Key words Eupatorium lindleyanum；compound Yemazhu capsules；flavonoids；LC．MS／MS This study was supported hy the Natural Science Foundation of Department of Science and Technology of Jiangsu Province (No．BK2004003)and the Public Foundation of Jiangsu Province(No．BM2004525) 野马追为菊科植物轮叶泽兰(Eupatorium lind— 1 材 料 leyanum DC．)的干燥地上部分，是江苏省特产中药 材。民间常作清热解毒药，有清肺止咳、化痰平喘、 1．1 药品与试剂 降血压之功