多柔比星壳聚糖微球的制备及其特性研究.pdfVIP

· 720· Herald of Medicine Vo1．26 No．7 July 2007 多柔比星壳聚糖微球的制备及其特性研究术 王增寿，胡 伟，张 华，朱光辉，陈 怡 (温州医学院附属第二医院药剂科，325027) [摘 要] 目的 以壳聚糖为载体材料，多柔比星为模型药物，制备脑内局部给药缓释微球。方法 以液体石蜡 为油相，L．抗坏血酸棕榈酸酯为交联剂，司盘-80为乳化剂，采用乳化化学交联技术制备多柔比星脑用微球。用动态透析 法检测微球的体外释放特性。结果 多柔比星／壳聚糖的质量比为1：9的载药微球形态良好，粒径分布较为均匀，平均 粒径为(9 41,4-2，43)la,m，载药量为(8．49±0，37)％，包封率为(70．56±4，23)％。体外释放具有良好的缓释效果。结 论 所优化的制备工艺稳定，适用于多柔比星壳聚糖脑用微球的制备。 [关键词] 多柔比星；壳聚糖；微球；缓释 [中图分类号] R979．1 [文献标识码] A [文章编号] 1004-0781(2007)07-0720-04 Study on Preparation and Properties of Doxorubicin-loaded Chitosan Microspheres WANG Zeng—shou，HU Wei，ZHANG Hua，ZHU Guang—hui，CHEN Yi(Department ofPharmacy，the Affiliated Second HospaaZ of Wenzhou Medical College。Wenzhou 325027，China) AB! RACT obj~tlve To study the preparation technique and release characteristic of doxorubicin．1oaded chitosan microspheres for the intracerebral local administration． Methods Using the liquid pala~n as the oil phase ．L．ascorbyl palmitate as the cross linking agent，and span-80 as the emu~ er．Doxorubicin—loaded chitosan microspheres were prepared by emulsion．chemical cross link technique Dynamic dialysis method was used to determine the releasing characteristic of microspheros in vitro Results Microspheres(doxombicin／chitosan=1：9) th a good shape and even particle size distribution were prepared．The average particle diameter was(9，41±2．43) m with a quantity of drug loading(8．49± 0．37)％．The proportion of entrapment was (70．56±4．23)％．The drug release in vitro had a satisfied effect of delayed release． Conclusion The optimized preparation technique is stable and has a high entrapment efficiency。suitable for the preparation of doxorubicin．1oaded chitosan microspheres for cerebral administration． KEY WORDS Dox