炭疽菌附着胞.pdfVIP

菌 物 学 报 25 4 660~665, 2006 Mycosystema 胶孢炭疽菌附着胞形成过程中核相的动态变化 1 2 1 2 万 瑶 张敬泽 * 马 青 谢关林 (1 植保资源与病虫害治理教育部重点实验室 西北农林科技大学植物保护学院 陕西杨凌 712100 2 浙江大学生物技术 研究所 杭州310029) 摘 要 通过DAPI 荧光染料染色观察胶孢炭疽菌Colletotrichum gloeosporioides 附着胞发育过程中的核相 动态变化 结果显示 第2 次有丝分裂发生的部位在分生孢子产生芽管的一端中 分裂后 最接近芽管的 一个子核移入芽管顶端 或通过芽管移入附着胞中 0.10g/mL 的三环唑可完全抑制附着胞中黑色素形成 但不影响核的分裂 三环唑处理 12 h 后 发生2 次有丝分裂数量约为73 而发生3 次有丝分裂的数量 约为23.9 % 绝大多数附着胞中是单核 双核数量小于5% 关键词 有丝分裂 三环唑 黑色素 中图分类号 Q939.96 文献标识码 A 文章编号 1672-6472 2006 04-0660-0665 Dynamic change of nuclear behavior in process of appressorium formation of Colletotrichum gloeosporioides WAN Yao1 ZHANG Jing-Ze 2* MA Qing1 XIE Guan-Lin2 1 ( Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, College of Plant Protection, Northwest Agriculture Forestry University, Yangling, Shaanxi 712100 2Biotechnology Institute, Zhejiang University, Hangzhou 310029) Abstract: Dynamic change of nuclear behavior was visualized during appressorial development by use of DNA specific fluorochrome, DAPI. It was demonstrated that the secondary nuclear division took place in one end of a conidium which produced the germ tube, and then the proximal daughter nucleus migrated forward to the germ tube apex or the appressorium. 0.10 g/mL tricyclazole completely inhibited melanin formation in the appressorium and failed to influence the nuclear division. After 12 h treatment in tricyclazole solution, two rounds of mitosis could be visualized, having a frequency about 73% of germinating spores observed, and three rounds of mitosis was also visible(23.9%). However, only one nucleus could be seen inside the appressorium in most cases, and 2 nuclei could be found in less