Improving thermal stability of enzymes by saltbridge strategy 清华大学概要1.ppt

* Foundation for the Author of National Excellent Doctoral Dissertation of China (200345) National Natural Science Foundation National 863 High-tech project (2007AA02Z201) National 973 fundamental project (2007CB714304) Company cooperation Thank you！ Acknowledgements: * Huimin YU, Jie Chen, Chang-Chun Liu, Jie Liu, Zhong-Yao Shen Institute of Biochemical Engineering Department of Chemical Engineering Tsinghua University, Beijing 100084 China 2011- 8-27 Improving thermal stability of enzymes by salt-bridge strategy：from single to network TWAS-ROESEAP-IB * Background- importance of enzyme’s stability Why salt-bridges? Where to construct the salt-bridge? How to construct the salt-bridge? From single to network of salt-bridges Outline * Industrial Biotechnology Demand of sustainable development USA-2030： Bioenergy: 20％ Biochemcals：25％ EU.-2010：Biochemicals： 10－20％ Government emphasis * OECD:“IB is the most promising technology for sustainable development of industry.” Industrial Biotechnology Company highlight * Raw material Products Bio-production Core of Industrial Biotechnology-- Biocatalysis Fermentation Raw material Purification Pipelines Product Waster water treatment Integration of Biotechnology and Chemical Engineering Cells or enzymes as catalysts to perform biocatalysis * Enzymes Metabolic pathway Gene Replication/ Transcription/Translation Phenotypes Immobilization Methods Enz. modification LJ Chien, et al, 2008 Core of Biocatalysis-Biocatalyst Immobilized enzymes Free cells Immobilized cells * Enzyme catalysts evolution Cell catalysts evolution Approaches to evolve enzymes * 《 Principles of Biochemistry 》 Characteristics of enzymes Flexibility of enzymes contributes to their high activity and specificity * Rigidity of enzymes contributes to their stability 《 Principles of Biochemistry 》 On web Characteristics of enzymes * Enzyme evolution Activity Specificity Stability On web Approaches to evolve enzymes * E