DAC对卵巢癌细胞株增殖及其MAGE、BAGE、GAGE基因表达的影响.pdfVIP

山东医药2007年第47卷第36期 DAC对卵巢癌细胞株增殖及其MAGE、 BAGE、GAGE基因表达的影响 韩风贤 。张师前“。周晓亮 。吴喜梅 。石 琰 (1海口市妇幼保健院，海南海口570216；2山东大学齐鲁医院) [摘要] 目的 探讨5-杂氮脱氧胞嘧啶(DAC)对卵巢癌细胞株SKOV3、A2780、COC1增殖及其肿瘤特异性抗 原MAGE、BAGE、GAGE基因表达的影响。方法 将对数生长期的卵巢癌细胞株与DAC共同培养，观察细胞生长 指数，利用流式细胞术检测细胞周期，RT-PCR技术检测卵巢癌细胞株在DAC作用前后MAGE、BAGE、GAGE基因 的表达。结果 DAC能使大多数巢癌细胞株阻滞在G。期，并使其 MAGE、BAGE、GAGE基因表达增加。结论 DAC能抑制卵巢癌细胞株的增殖，提高其肿瘤特异性抗原基因的表达。 [关键词] 5．杂氮脱氧胞嘧啶；卵巢肿瘤；肿瘤特异性抗原基因 [中图分类号] R737．31 [文献标识码] A [文章编号] 1002-266X(2007)36-0033-03 Effe~of 5．aza-2 ．deoxycytidine in proliferation of ovarian cancer eelI Iines and its effects on expression of MAGE，BAGE，GAGE gene HAN -xian ，ZHANG Shi-qian，ZHOUXiao—liang， -mei，SHI Yan (1 ikou Maternaf Hospital，Ha~ou 570216，P．R．China) Abstract：[Objective]To investigate the effect of 5-aza一2-deoxyeytidine(DAC)in proliferation of ovarian cancer cell lines and expression of tumor specific antigen MAGE，BAGE and GAGE．[Methods]Ovarian cancer cell lines were treated with DAC in CO-culture，the growth curves were observed，the cell cycle was detected by flow eytometry，the MAGE，BAGE，GAGE gene expression patterns were measured by RT—PCR before and after DAC treatment．[Results] The growth of most cells were stopped in GO phases after DAC treatment，the levels of MAGE，BAGE，GAGE gene expres— sions after DAC treatment were higher than before．[Conclusion]DAC has an inhibitive effect on the ovarian cancer cell lines，and call elevate the expression oftum or spec~c antigen gene． Key words：5 aza-2-deoxy eytidine；ovarian tumor；tum or spec~c an tigen gene MAGE、GAGE、BAGE基因是一种正常的基因， U／ml链霉素的RPMI 1640培养液中；当细胞长满培 它的表达受甲基化的调控。5-杂氮脱氧胞嘧啶 养瓶底的80％时，常规消化收集，一8O℃保存。参 (DAC)为一脱甲基制剂。2005年12月～2006年12 照文献…，将消化后的细胞以1×10。个接种于培养 月，我们将 DAC作用于卵巢癌细胞株 SKOV3、 瓶，贴壁后更换培养液并加入DAC(1 p,mol／L)，每 A2780、COC1，观察癌细胞的增殖及其 MAGE、 24 h换液并加DAC，连续培养，直至细胞生长达瓶底 GAGE、BAGE基因表达情况。现将结果报告如下。 的80％，消化收集细胞，一8