CIK细胞诱导及对K562细胞毒作用的研究.pdfVIP

Journal of Medical Forum Vo1．28 No．23 December 2007 ·17· CIK细胞诱导及对K562细胞毒作用的研究 胡杰英，蒋东霞，徐 虹，何 琳，买玲，宋永平，杨胜利 河南省肿瘤研究所 郑州市 450008 摘要 目的 体外诱导细胞因子诱导的杀伤细胞(CIK)，并研究其生物学活性。方法 从外周血分离单 个核细胞(PBMC)，经过细胞因子诱导、培养并扩增CIK细胞，以LAK细胞作比较，检测CIK的增殖能力，流式细 胞仪检测CIK细胞表面标志CD 、CD MTT法检测对I(562细胞系杀伤活性。结果 CIK细胞第二周进入快速 增殖期，到第21d扩增倍数超过120倍，CD[、CD 细胞扩增倍数达15倍以上；CIK对K562细胞的杀伤能力明 显优于LAK细胞。结论 CIK细胞是一种具有很强杀瘤活性的免疫活性细胞，具有临床应用前景。 关键词 细胞因子诱导的杀伤细胞(CIK)；K562细胞；表型；杀伤活性 中图分类号：R73—36 文献标识码：A 文章编号：1672—3422(2007)23．()017—02 Induction of Cytokine——induced Killer and Its Cytotoxic Effecton K5 62 Cells HU Jieying，JINAG Dongxia，XU Hong，et al Henan Tumor Institute，Zhengzhou 450003，China ABSTRACT objective To prepare cytokine—induced killer(CIK)in vitro，and study the biological activities of CIK．Methods Lymphocytes were isolated from peripheral blood and induced by cytokine．The proliferation of CIK was tested．The cells surface markers CD and CD of CIK were analyzed by FACS．The cytotoxic effect of CIK on K562 cells was measured by MTF．Results CIK cells quickly proliferated from the second week．and expanded more than 1 20一f0ld at the 2 1 th day af- ter induction．The CD3+and CDs6 cells expanded more than 15 folds ． The cytotoxic effect of CIK On K562 cells was obviously stronger than that of LAK cells．Conclusion CIK was a highly efficient cy— totoxic cells against tumors．and had clinical application potentials． KEY WoRDS CIK；K562 eell：Surface markers：Cytotoxity 肿瘤的生物治疗中，过继免疫治疗占有重要 Hepes 25nml，谷氨酰胺2ram，NaHCO 0．2％及2一巯 地位¨ 。CIK(CytokineInduced Killer)是将人外周 基乙醇10～mol／L，青链霉素双抗200U／ml，在37℃， 血单个核细胞(PBMC)在体外用多种细胞因子(如 饱和湿度及5％CO，条件下进行培养，3d换液一次。 抗CD3MeAb、IL一2、IFN—r等)共同培养一段