急性氨氮胁迫对于草鱼sod和hsp90基因表达及鳃部结构的影响.pdf

doi：10．7541／2013．21 THEEFFECT oFHIGH AMM oNIA CoNCENTRATIoN oN GILL STRUCTUREALTERNATIoN AND EXPRESSION oF AND 月 P90GENESIN GRASSCARP， CTENoPHARYNGoDoN IDELLA ZHOUXin ，DONGYun．wei，WANGFang andDONG Shuang．1in (1．TheKeyLaboratoryofMariculture，MinistryofEducation，FisheriesCollege，OceanUniversityofChina， Qingdao266003，China；2．StateKeyLaboratoryofMarineEnvironmentalScience，CollegeofOceanography andEnvironmentalScience，XiamenUniversiyt，Xiamen361005，China) Abstract：HistologicalandmolecularresponsesofgrasscarpCtenopharyngodonidellatohighconcentrationammonia exposurewerestudiedintllisstudybydeterminingthelethalconcentration．gillcollulra structure。na dexpressionpat． ternsofsodnadhsp90genes：Crra~scarpsweretreatedatfiveammoniaconcenrtations(50，72，104，15land220meL／) f0r24h，andthenthegil1tissueswerecollectedforhistologicalanalysis．Inaddition，threetissues(gi11，intestinenad liver)werecollectedtomeasuretheexpressionofsodandhsp90genes，Afterexposuretohigllconcenrtationofamino． nia，htearrna gementandstructuresofgillcellschangeddramatically．Edemanadfusionofsecondarylamellaewereob． se~ed。andtheedgeofeellandnucleuscouldnotbecleralydefined．Meanwhile．htetwogenes(sodnadhsp90)indif- ferenttissueswereunregulatedsignificantly,indicatinghtathighconcentrationofammoniacouldimlclairthecellular structuresna dinducehteexpressionofsrtessproteins．Theseresultsalsosuggestedthatsodandhsp90weresuitable biomarkersforammoniaexposureassessmentinrgasscarp． Keywords：Grasscarp；Ammoniaexposure；24hLCs0；Histologicaldamage；so hsp90 CLCnumber：X171．5 Documentcode：A ArticleID：1000-3207(2013)02-0321—08 Ammoniaisahighlytoxicwasteproductproducedby causeshyperventilation 【ljj ，hyper-excitability,coma， plantsand naimals【．Decompositionmediated by mi