半夏泻心汤与常规西医治疗幽门螺杆菌感染小鼠的疗效差异及机制分析.docxVIP

半夏泻心汤治疗幽门螺杆菌感染小鼠的疗效及机制分析摘要：目的：研究半夏泻心汤治疗幽门螺杆菌（Hp）感染小鼠的疗效及分子机制。方法：选择C57小鼠作为实验动物，随机分为对照组、模型组、干预A组、干预B组、干预C组，Hp灌胃后建立Hp感染模型，干预A组、干预B组、干预C组分别给予半夏泻心汤全方、清热解毒组分、扶正补益组分干预。干预后检测Hp根除情况、TLRs/NF-kB信号通路及下游炎症介质的表达量。结果：干预A组、干预B组、干预C组的幽门螺旋杆菌根除率均高于模型组且干预A组的幽门螺旋杆菌根除率均高于干预B组、干预C组，干预B组与干预C组间幽门螺旋杆菌的根除率无显著性差异；模型组小鼠胃黏膜中TLR2、TLR4、NF-kB、TNF-α、IL-6、IL-8的表达量显著高于对照组，干预A组、干预B组、干预C组小鼠胃黏膜中TLR2、TLR4、NF-kB、TNF-α、IL-6、IL-8的表达量显著低于模型组且干预A组小鼠胃黏膜中TLR2、TLR4、NF-kB、TNF-α、IL-6、IL-8的表达量显著低于干预B组、干预C组，干预B组与干预C组间胃黏膜中TLR2、TLR4、NF-kB、TNF-α、IL-6、IL-8的表达量无显著性差异。结论：半夏泻心汤全方及各组分均能根除Hp并抑制TLRs/NF-kB信号通路，且全方的疗效优于各组分。关键词：幽门螺旋杆菌；半夏泻心汤；Toll样受体；炎症介质Efficacy and mechanism analysis of BanxiaXiexin Decoction in treating Helicobacter pylori infectedmiceAbstract: Objective: To study the effect and molecular mechanism of BanxiaXiexin Decoction in treating Helicobacter pylori (Hp) infected mice. Methods: C57 mice were collected as experimental animal and were randomly divided into control group, model group, intervention A group and intervention B group and intervention C group. Hp infection model was made by Hpintragastric administration, intervention A group and intervention B group and intervention C group were given BanxiaXiexin whole decoction, qingrejiedu fraction, fuzhengyiqi fraction. After the intervention, tHp eradication, the expression of TLRs/NF-kB signaling pathway and inflammatory mediators were detected. Results: Hperadication rat of intervention A group, intervention B group, intervention C group were higher than that of model group and Helicobacter pylori eradication ratesof intervention group A were higher than intervention B group and intervention C group, Helicobacter pylori eradication rates ofintervention B group and intervention C group had no significant difference; theexpression of TLR2, TLR4, NF-kB, TNF-α, IL-6, IL-8 of intervention A group, intervention B group, intervention C group were lower than that of model group, theexpression of TLR2, TLR4, NF-kB, TNF-α, IL-6, IL-8 of intervention A groupwere lower