当归、黄芪注射液对鸡胚绒毛尿囊膜血管生成的影响.pdfVIP

第3O卷第12期2007年12月 北京中医药大学学报 Vo1．30 No．12 Dec．2007 Journal of Beijing University of Traditional Chinese Medicine · 中医药实验研究· 当归、黄芪注射液对鸡胚绒毛尿囊膜血管生成的影响 杨丽霞 卢雯玲 王志程 牛 洁 刘铜华件 魏道武 (1北京中医药大学 北京100029；2甘肃中医学院) 摘要：目的 探讨当归、黄芪注射液对鸡胚绒毛尿囊膜(chorioallantoic membrane，CAM)血管生成的 影响。方法 种蛋在37．8℃孵育7 d后制备CAM模型，当归、黄芪注射液及其不同配比滴加于 CAM表面的载体上，继续孵育3 d，观察鸡胚存活状况，然后制备鸡胚CAM标本，解剖显微镜下计 数新生毛细血管数目。结果 当归、黄芪注射液及其不同配比均具有促进鸡胚CAM血管生成作 用，与生理盐水对照组相比有显著性差异(P0．05)，除当归与黄芪1：5，其他各组药效低于表皮生 长因子(EGF)(P0．05)。结论 当归注射液、黄芪注射液及其不同配比具有显著促进鸡胚CAM 血管生成作用，其可能的机制是血管内皮细胞的增殖与迁移。 关键词：血管生成；鸡胚绒毛尿囊膜；当归注射液；黄芪注射液 中图分类号：R285．5 Influence of Danggui Injection and Huangqi Injection on angiogenesis of chick chorioallantois YANG Li—xia，LU Wen—ling，Wang Zhi—cheng，et a1． (BeOing University of Chinese Medicine，Beijing 100029) Abstract：Objecfive To investigate the influence of Danggui Injection and Huangqi Injection on the angiogenesis of chick chorioaUantois．Methods The chick chorioallantois model was established by incubating fertilized eggs at 37．8~C for 7 days,Danggui Injection and Huangqi Injection with various ratios were dropped on the cartier of chorioallantois and then the eggs were continuously incubated for 3 days．The survival status of chick was observed and the chick chorioallantois sample was prepared，and then the number of new capiUaries Will8 counted by using anatomic microscope．Results Danggui Injection and Huangqi Injection with va~ous ratios all promoted the angiogenesis of chick chorioallantois with a significant difference(P0．05)compared tII normal saline solution．Except of ratio of 1 to 5 between Danggui Injection and Huangqi Injection，other ratios had lower efficacies than epidermal growth factors(EGF，P0．05)．Conclusion Danggui Injection and Huangqi Injection with various ratios have a significant pro