植物药瓜子金总皂苷的含量测定及其纯化工艺研究.doc

  植物药瓜子金总皂苷的含量测定及其纯化 工艺研究# 王洪兰1，张亚楠2，陈丽红1，李祥1** 5 10 15 20 25 30 35 40 （1. 南京中医药大学药学院，南京 210046； 2. 上海第二军医大学航海与潜水医学教研室，上海 200433） 摘要：目的：建立瓜子金总皂苷含量测定的方法学，研究瓜子金中总皂苷的纯化工艺。方法： 采用紫外可见分光光度法测定瓜子金中总皂苷含量，考察大孔树脂吸附色谱富集纯化瓜子金 总皂苷的工艺。结果：瓜子金总皂苷在测定范围内均显示较好的线性关系（r=0.9997）；精 密度、重复性、稳定性试验中 RSD 值均小于 3.00%；回收率平均值是 99.51%；大孔树脂纯化 试验表明 70%乙醇洗脱部位总皂苷含量达 71.68%。结论：本研究所建立的方法快速、准确， 可测定瓜子金中总皂苷的含量，同时大孔树脂可有效的富集纯化总皂苷，为远志属药用植物 瓜子金资源的综合利用提供理论支撑。 关键词：中药化学；瓜子金；总皂苷；含量测定；大孔吸附树脂 中图分类号：请查阅《中国图书馆分类法》 Study on determination and purification of total saponins from Polygala japonica WANG Honglan1, ZHANG Yanan2, CHEN Lihong1, LI Xiang1 (1. Nanjing University of Chinese Medicine, Nanjing 210046; 2. Department of Diving medicine, The Second Military Medical University, Shanghai 200433) Abstract: OBJECTIVE: To establish a method on determination and purification of total saponins from Polygala japonica. METHOD: Content of total saponins from Polygala japonica was determined by the UV. The purification of total saponins was determined by macro-porous resin adsorption chromatography. RESULT: The amount of total saponins showed good linear relationship in the range of determination (r=0.9997). RSD of the precision, repeatability and stability were all less than 3.0%. The average recovery rate was 99.51%. The content of 70% ethanol elution fraction was 71.68% in macroporous resin purification of total saponins enrichment experiment. CONCLUSION: The method established in the study is rapid, accurate and can be applied to directly determination and purification of total saponins from Polygala japonica, and may provide theoretical support for a comprehensive and efficient use of the resource of Polygala japonica. Key words: Traditional Chinese Medicinal Chemistry; Polygala japonica; total saponins; content determination; macro-porous resin adsorption chromatography 0 引言 瓜子金始载于《植物名实图考》，又名小远志、地藤草、银不换，是远志科远志属植物 瓜子金（Polygala japonica Houtt.）的全草，主要分布于我国南方各省[1]。瓜子金味苦、微辛、 性平，能镇咳祛痰、益智安神、活血散瘀，民间应用十分普遍且疗效显著。瓜子金中的化学 基金项目：江苏高校优