孑遗植物格氏栲居群遗传多样性.docVIP

  孑遗植物格氏栲居群遗传多样性# 刘金福1，林义君1，潘东明2，洪伟1，何中声1，吴则焰3，郑世群1* （1. 福建农林大学林学院，福州 350002； 5 10 15 20 25 30 35 40 45 2. 福建农林大学园艺学院，福州 350002； 3. 福建农林大学生命科学院，福州 350002） 摘要：采用 ISSR 分子标记技术对壳斗科属格氏栲 18 个居群共 74 个样品的遗传多样性水平 和遗传结构进行研究。10 个引物共获得 113 条清晰扩增位点，其中多态性位点 98 个。POPGENE 分析结果表明，格氏栲居群具有丰富的遗传多样水平，即多态位点百分率 PPB =86.73%、Nei’ s 基因多样度 H=0.2791、Shannon’s 信息指数 I=0.4246。遗传分化系数 Gst 为 0.4623，基 因流 Nm 为 0.5816，表明格氏栲居群间和居群内均存在比较显著的遗传分化。UPGMA 聚类分 析反映出格氏栲居群遗传距离与地理距离存在正相关性。应加大对遗传多样性高的格氏栲居 群保护力度，加强格氏栲居群间基因交流，以最大限度地保存格氏栲资源的遗传多样性。 关键词：森林生态学；格氏栲；ISSR；遗传多样性；遗传结构；保护对策 中图分类号：S664.9 Genetic Diversity for Castanopsis Kawakamii Populations LIU Jinfu1, LIN Yijun1, PAN Dongming2, HONG Wei1, HE Zhongshen1, WU Zeyan3, ZHENG Shiqun1 (1. Forestry College of Fujian Agriculture and Forestry University, FuZhou 350002; 2. Horticulture College of Fujian Horticulture College of Fujian Agriculture and Forestry University, FuZhou 350002; 3. Life Sciences College of Fujian Agriculture and Forestry University, FuZhou 350002) Abstract: ISSR molecular markers used Fagaceae genitives 18 levels of genetic diversity of populations and genetic structure were studied. 10 primers used in 18 populations of 74 samples were amplified total of 113 clear amplification sites，98 of them polymorphic. POPGENE analysis showed that Castanopsis kawakamii populations rich in genetic diversity levels（percentage of polymorphic PPB = 86.73%，Neis gene diversity H = 0.2791，Shannons information index I = 0.4246）. Coefficient of genetic differentiation Gst was 0.4623，gene flow Nm to 0.5816， thats populations and within populations of Castanopsis kawakamii were all exist relatively significant genetic differentiation.UPGMA cluster analysis shows Castanopsis kawakamii genetic distance and geographical distance was positively correlated. Based on population genetics and Castanopsis kawakamii ecology research results ， should increase the genetic diversity of populations of Castanopsis kawakamii high protection，and strengthen Castanopsis kawakamii gen