膜技术及其在分析样品处理中的应用.ppt

* Here is the quintessential Millipore in the areas of ASP We have a solid offering of filtration based products – centered around the traditional way to do a prep Putting a filter in a glass holder Attaching a syringe onto a filter And filtering Not a lot of technological innovation for quite a while This is a business that we have milled – tried to squeeze from a market perspective The legacy, the bank, the ATM machine – all words used to describe All these leads to a business that historically has modest single digit growth and thought to be in the mature /refresh stage of PLM With this position there are risks of being replaced or technology becoming obsolete – an example is the uniprep and or the Dionex online syringeless auto sampler vial Even though UHPLC is a form of HPLC, sample preparation needs and mobile phase preparation needs for UHPLC are different than that for HPLC. The main reasons for this are listed above. * 那市面上有各种各样的过滤器，过滤膜，是不是都一样呢？我这里有张图，上面是聚丙烯材质的，下面是特富龙材质的。我们可以清楚地看到聚丙烯的过滤后的流动相显著地增加了UPLC系统的背压，而特富龙基本维持。因此可以看出，使用不同材质的滤器，所得到的过滤效果也不相同。选择适合的过滤器是很重要的。 * UHPLC Sample requirements for UHPLC are very similar to that for HPLC meaning that a sample free of particulate impurities is needed for HPLC. Removal of as many interfering components is crucial as that improves separation, background signal and quantitation capability This graph clearly shows that not all 0.2 um membranes are created equal. The data shows that when comparing Nylon and PES membranes both the vendor membranes show very similar particle retention ( 95%) whereas in case of PTFE and PVDF membranes, Vendor A retains greater than 95% particles whereas the vendor B membranes can only retain about 80% of particles indicating that these filters allow 20% of the particles to go through the syringe filters. In these experiments about 5 ml of Polystyrene latex suspension (0.05%w/v) containing latex beads of 0.3 um (300 nm) were filtered through different syringe filters and the filt