李梨平-标记免疫检测技术新进展.pptVIP

* * These three subunits (α, βA, βB) are products of separate genes located on chromosomes 2 (α and βB subunit) and 7 (βA subunit) There is 23-27% amino acid homology between the α subunit and β subunit and 64% homology between the β subunits. Each subunit is initially assembled as a large pre-pro-protein which possess a N-terminal signal peptide (a pro-region needed for the accurate folding and dimerization of the subunits and which targets the polypeptide to the endoplasmic reticulum) and the mature C-terminal domain. Proteolytic partial cleavage of pro-region makes it biologically active. Proteolytic partial cleavage of pro-region makes it biologically active. Partial processing of proforms and glycosylation pattern on C-terminus of the subunit will give rise to different isoforms of inhibin and have been reported in bovine and human plasma * * 血清抑制素A 在卵泡早期处于低水平, 排卵后上升, 在黄体中期达最大值。卵泡期血清抑制素A 与雌二醇呈正相关, 提示此期抑制素A 主要由优势卵泡产生, 此后黄体期抑制素A 与孕酮水平呈正相关, 在黄体中期分泌至高峰, 伴随着黄体的退化而逐渐下降, 说明抑制素A 在黄体期主要同黄体细胞产生, 与E2 一起在黄体卵泡期过渡期下降, 反馈于垂体, 引发了FSH 分泌的增加, 正是这一FSH 分泌小峰对该期募集有重要作用。因此在外源性FSH 的作用下, 主要由黄体分泌的INHA 与卵巢负调节FSH 分泌的逐渐释放有关。因此, 对于调节女性卵泡的发育, 生殖方面起了重要作用 * FTS（First Trimester Screening）：孕早期联合筛查，NT+PAPPA+βhCG；IPS（Integrated Prenatal Screening）：整合筛查，NT+PAPPA/1T, AFP+βhCG+uE3+Inhibin A/2T；SIPS（Serum Integrated Prenatal Screening）：血清整合筛查，PAPPA/1T, AFP+βhCG+uE3+Inhibin A/2T；Qud（Quadruple Screening）：孕中期四联筛查，AFP+βhCG+uE3+Inhibin A * * * * * * * 抑制素A水平放映了IVF-胚胎移植治疗后黄体数量；单胎组与多胎组HCG水平不同，但抑制素A水平无差异，反映了妊娠早期（胚胎移植后第16天）抑制素A水平与胎盘无关，与黄体功能有关。 * patients with early pregnancy bleeding and an intrauterine sac with fetal cardiac activity (threatened abortion) progressing till term have higher inhibin A than those with threatened abortion but whose pregnancy failed later, This finding may add significant prognostic information for predicting the pregnancy outcome. * 妊娠期高血压疾病是妊娠期特发性疾病，其中子痫前期是该疾病发生的常见类型，迄今为止仍为孕产妇及围生儿死亡的重要原因之一。 * 中国人群的数据： Second-trimester inhibin A levels in 93 normal controls and in 31 pregnancies