三种金黄色葡萄球菌显色培养基检测效果比较.PDFVIP

微生物学通报 AUG 20, 2009, 36(8): 1105~1109 Microbiology © 2009 by Institute of Microbiology, CAS tongbao@ 研究报告 三种金黄色葡萄球菌显色培养基检测效果的比较 1,2 1* 1 1 1 黄汝添 吴清平 张菊梅 郭伟鹏 吴许文 (1. 510070) (2. 510070) 摘 要: 本文研究 2 种国外主流的金葡菌显色培养基和广东环凯微生物科技有限公司生产的 PEN-TCF 检测金葡菌的效果。通过研究 3 种产品对多种金葡菌菌株的回收率、最低检出限以及检 测人工污染食品的回收率、特异性和抗干扰能力比较其检测效果。结果表明3 种培养基的最低检 出限相近, 显色培养基A 和PEN-TCF 的回收率较高, 但抗干扰能力较低; 显色培养基B 回收率稍 低, 而抗干扰能力较强; PEN-TCF 和培养基B 的特异性更优于培养基A 。 关键词: 金黄色葡萄球菌, 显色培养基, 检测比对 Comparison of the Detection Effect of Three Chromogenic Media of Staphylococcus aureus HUANG Ru-Tian1,2 WU Qing-Ping1* ZHANG Ju-Mei1 GUO Wei-Peng1 WU Xu-Wen1 (1. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China) (2. Guangdong Huankai Microbial Science and Technology Corporation, Guangzhou, Guangdong 510070, China) Abstract: Two current main chromogenic media products of abroad and the newly developed PEN-TCF from Guangdong Huankai Microbial Sci. Tech. Corporation were compared in this study. Their re- covery of different Staphylococcus aureus strains, minimum detection limits and the recovery, specificity and anti-distubance of detecting artificial contaminated samples were studied. The results showed that 3 media had similar minimum detection limits, Chromogenic Media A and PEN-TCF had better sensitiv- ity but worse anti-distubance, while Chromogenic Media B had better anti-distubance yet lower sensi- tivity, and Media B and PEN-TCF w